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Cas12aUltra_5XNLS

Plasmid

#218775

Purpose

His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

Depositor

Ross Wilson

Article

Sahu et al bioRxiv 2024.07.14.603391

Insert

5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a

Use

CRISPR

Tags

6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

Expression

Bacterial

Mutation

Nucleotides mutations were made to encode for arg…

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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p5154

Plasmid

#175390

Purpose

High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

Depositor

Michael Wiles

Article

Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

Type

Empty backbone

Use

Mouse Targeting; Cloning vector, to be used to de…

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pT18mobsacB

Plasmid

#72648

Purpose

sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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330-Plakoglobin-Myc

Plasmid

#32228

Depositor

Kathleen Green

Article

Palka et al J Cell Sci. 1997 Oct;110 ( Pt 19):2359-7

Insert

Plakoglobin (JUP Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

human beta-actin

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowR

Plasmid

#132668

Purpose

Expresses ShadowR in mammalian cells

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2019 Aug 19;9(1):12072. doi: 10

Insert

ShadowR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFast-hRad1

Plasmid

#222471

Purpose

Expresses hRad1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad1 (RAD1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry

Plasmid

#207668

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP)

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

MCP-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CMV BARK p2A mCherry

Plasmid

#117689

Purpose

CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

Tags

HA / FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3

Plasmid

#73021

Purpose

Expresses BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-YapS127A

Plasmid

#46049

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

Flag tag

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
CMV BARK D110A p2A mCherry

Plasmid

#117690

Purpose

CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Tags

HA / FLAG

Expression

Mammalian

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-cMyc

Plasmid

#46970

Purpose

lentiviral expression of c-Myc

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCI NL4-3 Nef-HA-WT

Plasmid

#24162

Depositor

Warner Greene

Article

Geleziunas et al Nature. 2001 Apr 12. 410(6830):834-8.

Insert

Nef (nef HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-hGPR56-IRES-GFP

Plasmid

#52297

Purpose

Expresses human GPR56 and GFP in mammalian cells.

Depositor

Christopher A Walsh

Article

Bae et al Science. 2014 Feb 14;343(6172):764-8. do

Insert

Human GPR56 coding sequence (ADGRG1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFMRPiso1

Plasmid

#48690

Purpose

Expresses hFMRP iso 1

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 (FMR1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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Lyric-mNeonGreen

Plasmid

#226562

Purpose

Encodes Lyric protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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phage UbiC tagRFP-T-DDX6

Plasmid

#119947

Purpose

Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

tagRFP-T

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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AcGFP1-C1

Plasmid

#54607

Purpose

Localization: C1 Cloning Vector, Excitation: 475, Emission: 505

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19_HDRT_TRAC_CD19-CAR

Plasmid

#183473

Purpose

Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

Depositor

Dimitrios Wagner

Article

Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

Inserts

TRAC (TRAC Human)
CD19-specific CAR transgene insert
TRAC (TRAC Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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