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pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDD132

Plasmid

#119885

Purpose

Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

BbaJ23104_EYFP

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-Cre

Plasmid

#235151

Purpose

Expresses Cre recombinase under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

Cre

Use

AAV

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKFSS1_2

Plasmid

#118227

Purpose

Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-mTFP1

Plasmid

#102635

Purpose

In vitro expression of mTFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTFP1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-CyPet

Plasmid

#102636

Purpose

In vitro expression of CyPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

CyPet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(1)

Plasmid

#136060

Purpose

G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCk3

Plasmid

#136697

Purpose

Acceptor plasmid for odd level position 3, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL0_142 [EilR-PjexD]

Plasmid

#199026

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

EilR-PjexD

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pWT016i

Plasmid

#96856

Purpose

theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTorPE-GR-GECO1.1

Plasmid

#42198

Depositor

Robert Campbell

Article

Hoi et al J Am Chem Soc. 2012 Dec 26.

Insert

GR-GECO1.1

Tags

6His

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pDV05.2_pEF1a-iRFP720_secreted

Plasmid

#200409

Purpose

Expresses a secreted version of iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (secreted)

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_NT_AmpR

Plasmid

#119144

Purpose

Encodes non-targeting sgRNA

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

non-targeting sgRNA

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Mutation

Y39Stop

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSLAX13 HA (CT#233)

Plasmid

#14027

Depositor

Cliff Tabin

Article

Logan et al Methods. 1998 Apr . 14(4):407-20.

Type

Empty backbone

Tags

3x HA

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICS NU-MT P2A

Plasmid

#210129

Purpose

Detect the Nucleus-Mitochondria contact

Depositor

Tito Calì

Article

Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

Insert

SPLICS NU-MT P2A

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB2989

Plasmid

#63636

Purpose

pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2875

Plasmid

#63640

Purpose

pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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