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We narrowed to 14,439 results for: eng

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Showing: 5181 - 5200 of 14439 results

  1. pcDNA3-sACE2-WT(732)

    Plasmid
    #154098
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154098-plasmid-map-sequence-id-304580

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  2. pcDNA3-sACE2v2-Fc(IgA1)

    Plasmid
    #145157
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgA1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145157-plasmid-map-sequence-id-292593

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  3. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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  4. pCEP4-intron-BaLgp160

    Plasmid
    #100918
    Purpose
    Mammalian expression plasmid for Env from the BaL HIV-1 isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) Env with upstream intron
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100918-plasmid-map-sequence-id-189993

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  5. CIAR_pcDNA5/FRT/TO

    Plasmid
    #86502
    Purpose
    Expresses CIAR in mammalian cells. Used to generate Flp-In stables.
    Depositor
    Dustin Maly
    Publication
    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIAR (SOS1 Human)
    Use
    Frt
    Expression
    Mammalian
    Mutation
    T968L in SOScat
    Promoter
    CMV (tet operator)
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86502-plasmid-map-sequence-id-171532

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  7. pGGD_RT_SRDX

    Plasmid
    #136976
    Purpose
    Goldengate/Greengate module EAR-repression domain
    Depositor
    Klaus Theres
    Insert
    SRDX
    Expression
    Bacterial
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136976-plasmid-map-sequence-id-272093

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  8. pGGD_RT_li_Tq2CFP

    Plasmid
    #136971
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Expression
    Bacterial
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136971-plasmid-map-sequence-id-269106

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  9. pGGB_RT_Tq2CFP_li

    Plasmid
    #136965
    Purpose
    Goldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Expression
    Bacterial
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136965-plasmid-map-sequence-id-269100

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  10. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid
    #234953
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Publication
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234953-plasmid-map-sequence-id-467731

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  12. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Publication
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  13. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

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  14. pSCIP-hCD69-TdTomato

    Plasmid
    #154095
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    [5HA]-P2A-tdTomato-[3HA]
    hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154095-plasmid-map-sequence-id-307109

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  16. pCEP4-DU422gp140-TM

    Plasmid
    #123251
    Purpose
    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (DU422) Env
    Tags
    CD5 leader peptide and Linker (with 6his tag) and…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123251-plasmid-map-sequence-id-235207

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  20. pSG5-FLAG-mEKLF

    Plasmid
    #67833
    Purpose
    Expression of FL-EKLF driven by SV40 promoter
    Depositor
    James Bieker
    Publication
    Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EKLF (Klf1 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Full-length mEKLF is aa 20-376; amino acid 19 is …
    Promoter
    SV40
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5181 - 5200 of 14439 results