We narrowed to 5,206 results for: paav

1. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

   Plasmid

   #176836

   Purpose

   Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Tet operator (tetracycline inducible expression)

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. eMscL G22S

   Plasmid

   #107455

   Purpose

   eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Mutation

   Bearing a glycine to serine substitution at posit…

   Promoter

   human synapsin 1

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hSyn-DIO-somBiPOLES-mCerulean

   Plasmid

   #154951

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CaMKII-somBiPOLES-mCerulean

   Plasmid

   #154948

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa(0.4)

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC13P-iCLHN

   Plasmid

   #66580

   Purpose

   CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Nanoluc-HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

   Plasmid

   #179435

   Purpose

   Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-coreHS4-mch (SC)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV2_hSyn_Aurora_Citrine

   Plasmid

   #98217

   Purpose

   Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV2_hSyn_iChloC_CA_Citrine

   Plasmid

   #98220

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iChloC_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, C128A, T159C, D156N

   Promoter

   human synapsin

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

   Plasmid

   #179434

   Purpose

   Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-HS4-mch (SH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hSyn-GtACR2-ChRmine-tandem-eYFP

    Plasmid

    #192580

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    EYFP- soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179436

    Purpose

    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    HS4-TRE-cit-HS4-mch (DH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179437

    Purpose

    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    coreHS4-TRE-cit-coreHS4-mch (DC)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV2_hSyn_Phobos_CA_Citrine

    Plasmid

    #98218

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179429

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TET1CD-CRY2-EGFP

    Plasmid

    #82555

    Purpose

    Encodes TET1 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    TET1 (TET1 Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid

    #98219

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV9:cTNT::3Flag-hYAP S127A

    Plasmid

    #86558

    Purpose

    To produce cardiac specific adeno-associated virus expressing activated human YAP.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

    Insert

    YAP (YAP1 Human)
    

    Use

    AAV

    Tags

    3Flag

    Expression

    Mutation

    Serine 127 was mutated into Alanine, to activate …

    Promoter

    cTNT

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188747

    Purpose

    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid

    #163701

    Purpose

    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Inserts

    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    3x-HA and P2A-tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE3G

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge