We narrowed to 29,593 results for: REP

1. pcDNA 3.1 SIRT1-FL

   Plasmid

   #105670

   Purpose

   Mammalian expression construct of mouse SIRT1 full length isoform

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hPGRN

   Plasmid

   #213682

   Purpose

   Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

   Depositor

   Thomas Kukar

   Article

   Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

   Insert

   Human Progranulin (hPGRN) (GRN Human)
   

   Use

   AAV

   Tags

   Twin-Strep tag and V5 tag (after signal peptide)

   Expression

   Mutation

   Promoter

   cytomegalovirus enhancer/chicken β-actin promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-BEACON2

   Plasmid

   #171698

   Purpose

   Expresses BEACON2 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

   Insert

   BEACON2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_W98Y/W104A/Y130F

   Promoter

   CMV

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-dV-IgG1/κ

   Plasmid

   #52213

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin kappa constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-102.1F10-IgM/λ

   Plasmid

   #61880

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScalps_Puro_mTet2 catalytic domain

   Plasmid

   #79554

   Purpose

   Expression of catalytic domain of mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   aa990-1912

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-VTKS1-hChR2-tBFP

   Plasmid

   #178706

   Purpose

   AAV vector for transgene expression of hChR2-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   hChR2-tBFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. MSCV-human Igf2bp2-IRES-GFP

   Plasmid

   #91890

   Purpose

   Retroviral expression of human Igfbp2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Igf2bp2 (IGF2BP2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-BSD-tet-mEGFP-MIB1

    Plasmid

    #140241

    Purpose

    Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Tet-inducible mEGFP-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRetroX-Tight_A-CREB_PGK-tdTomato

    Plasmid

    #154264

    Purpose

    Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    dominant negative CREB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight/tetO

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST scFv [N100/13]

    Plasmid

    #190521

    Purpose

    Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GST (Schistosoma japonicum) recombinant scFV
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Parkin-IRES-zeo

    Plasmid

    #61727

    Purpose

    Expression of human Parkin fused to mCherry

    Depositor

    Douglas Green, Stephen Tait

    Article

    Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pLV 4xSTAR-mTagBFP2-blast

    Plasmid

    #125239

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTagBFP2 (codon optimized)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

    Plasmid

    #204696

    Purpose

    This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of the amino acids 355-505 of NUT

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

    Plasmid

    #204695

    Purpose

    This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-NUT(MIN) (BRD4, NUTM1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of majority of NUT, retaining only amino…

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Anti-GFP [N86/34R]

    Plasmid

    #220419

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVITRO1-dV-IgG1/λ

    Plasmid

    #52214

    Purpose

    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid

    #100597

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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