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Anti-VIP [L108/92R]

Plasmid

#177453

Purpose

Mammalian Expression Plasmid of anti-VIP (Human). Derived from hybridoma L108/92.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VIP (Homo sapiens) recombinant mouse monoclonal antibody (VIP Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139097

Purpose

Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dxCas9-NG

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-VAPA [N479/22R]

Plasmid

#188181

Purpose

Mammalian Expression Plasmid of anti-VAPA (Rat). Derived from hybridoma N479/22

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAPA (Rattus norvegicus) recombinant mouse monoclonal antibody (Vapa Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VAPA [N479/12R]

Plasmid

#199401

Purpose

Mammalian expression plasmid of Anti-VAPA [N479/12R] (Rat). Derived from hybridoma N479/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VAPA (Rat) recombinant mouse monoclonal antibody (Vapa Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

Plasmid

#91881

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pNL-CEF-IgA1

Plasmid

#187009

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-GFP

Plasmid

#178714

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

Plasmid

#154263

Purpose

Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin scFv [L11A/41]

Plasmid

#206722

Purpose

Mammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BEACON1

Plasmid

#171697

Purpose

Expresses BEACON1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

Insert

BEACON1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

hAPOBEC3A_W104A/Y132D

Promoter

CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#182072

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgATXN1L-1

Plasmid

#74962

Purpose

Cas9 + sgATXN1L-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgATXN1L-2

Plasmid

#74963

Purpose

Cas9 + sgATXN1L-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

Plasmid

#139091

Purpose

Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dSpCas9-NG-VPR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Neuroligin-2 scFv [L107/90]

Plasmid

#206763

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-hTSC1-T2A-Bsd

Plasmid

#193199

Purpose

Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

TSC1 (TSC1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD3

Plasmid

#170848

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD4

Plasmid

#170849

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD5

Plasmid

#170850

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD6

Plasmid

#170851

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge