We narrowed to 5,482 results for: pcas

1. pCMV_T7-PE2-Nuclease

   Plasmid

   #171997

   Purpose

   Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CMV_T7-Cas9-RT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-PE

   Plasmid

   #162796

   Purpose

   All-in-one prime editor piggyBac transposon

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL11060

   Plasmid

   #68264

   Purpose

   Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRY2clust-mCherry-RANK

   Plasmid

   #208612

   Purpose

   Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)

   Depositor

   Tomohiro Ishii, Takao Nakata

   Article

   Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

   Insert

   CRY2clust-mCherry-RANK (Cry2 Mouse, Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC-BAR-YR

   Plasmid

   #61766

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Bar gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. RpH-ILV-FRB

   Plasmid

   #225137

   Purpose

   RpH-ILV probe strongly colocalises with lysosomal markers with FRB

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p323.hSyn.Halo-mFMRP.Wpre

   Plasmid

   #185909

   Purpose

   Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

   Depositor

   Young J Yoon

   Article

   Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

   Insert

   HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   hSyn (human Synapsin promoter)

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AID-NG-CBE

   Plasmid

   #227416

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   AID-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AID-N51A-NG-CBE

    Plasmid

    #227417

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    AID-N51A-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-mitf

    Plasmid

    #69801

    Purpose

    Used to make mitf knockout porcine cell line

    Depositor

    Jianguo Zhao

    Article

    Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

    Inserts

    mitf-sgRNA
    SpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-Actin and U6

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Use

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

    Plasmid

    #160624

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2:VPR:Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173862

    Purpose

    Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

    Plasmid

    #160633

    Purpose

    TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31-RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

    Plasmid

    #149277

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid

    #68218

    Purpose

    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc / Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos / 35S / 35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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