We narrowed to 22,280 results for: fus
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pLVX_TRE3G_DD::mCherry
Plasmid#108679PurposeTunable system for controlled gene expression and protein stability in mammalian cellsDepositorInsertDestabilizing Domain (DD) mCherry fusion protein
UseLentiviralTagsDD::mCherry fusion proteinExpressionMammalianMutationPromoterDoxycycline inducibleAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3mut-MAPK
Plasmid#29690PurposeGFP reporter protein fused to three mutated GSK3 phosphorylation sitesDepositorInsertGFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
UseTagsFlag and Flag-EGFPExpressionMammalianMutationrecognition sites only, and E3 ligase site, GSK3 …PromoterSP6Available sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGWB501
Plasmid#74843PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLTR.gp140/EGFP.Revdelta38/DsRed
Plasmid#115775PurposeHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.DepositorInsertEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
UseTagsEGFP; dsReDExpressionMammalianMutationgp140 uncleaved (1190-91aa, KR > TG; truncated…PromoterHIV-1 (LTR)Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-EGFP
Plasmid#174186PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertEGFP with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro DEST (670-1)
Plasmid#17293Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGWB402
Plasmid#74796PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/BDD-FVIII
Plasmid#41035DepositorInsertB domain deleted FVIII (F8 Human)
UseTagsExpressionMammalianMutationB domain deletionPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP DEST (736-1)
Plasmid#19732Purpose3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAi; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(WT)-EGFP
Plasmid#89633PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiRhoA2G
Plasmid#40179DepositorInsertRhoA Biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-AF8
Plasmid#117803Purposemammalian expression of GFP-tagged human centromere protein ADepositorInsertCENP-A (CENPA Human)
UseTagsGFPExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-cathepsinD-YMH
Plasmid#182644PurposeMammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitopeDepositorInsertcathepsinD
UseRetroviralTagsHis6-tag, TS site from proCCK, and myc-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mKate2-Rab11
Plasmid#174463PurposeExpress ｍKate2 with recycling endosome-targeting sequence in mammalian cellsDepositorInsertmKate2 with recycling endosome-targeting sequence
UseTagssmall GTPase (Rab11)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-ATG5
Plasmid#13095DepositorInsertATG5 (Atg5 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmApple
Plasmid#191259PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmAppleDepositorInsertLSSmApple
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL-SaKKH-BE3
Plasmid#85170PurposeExpresses SaKKH-BE3 in mammalian cells [Edit]DepositorInsertSaKKH-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Plasmid#121184PurposeOsTIR1 introductionDepositorInsertCMV-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_206
Plasmid#96920Purposefor SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffoldDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-EGFP
Plasmid#175827PurposeExpress EGFP with microtubule-targeting sequence in mammalian cellsDepositorInsertEGFP with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCpf1-BE
Plasmid#107685PurposeExpresses dLbCpf1-BE in mammalian cellsDepositorInsertdLbCpf1-BE
UseTagsExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBK-VQR-BE3
Plasmid#85171PurposeExpresses VQR-BE3 in mammalian cellsDepositorInsertVQR-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSL3402 (pTnsABf_Pse)
Plasmid#200901PurposeExpresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.DepositorInsertPseTnsA-TnsB
UseTagsInternal NLS between TnsA-TnsB fusionExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(5mut)-mGreenLantern
Plasmid#219661PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (5 mutations) fused to mGreenLantern
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(3mut)-mGreenLantern
Plasmid#219662PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (3 mutations) fused to mGreenLantern
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
