We narrowed to 12,972 results for: BASE
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Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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ZJOM157
Plasmid#133640PurposeIntegration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS1222
Plasmid#63791PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection markerDepositorInserteGFP-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseGFP-Twin-Strep tagPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-MYO1C
Plasmid#227305PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.DepositorInsertsgRNA Targeting N-terminus of MYO1C (MYO1C Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SPW-RHH
Plasmid#190630PurposeBacterial expression of genetically encoded protease indicator SPW-RHHDepositorInsertSPW-RHH
ExpressionBacterialAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX
Plasmid#188991PurposeA plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6020nes
Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
TagsXFPExpressionMammalianAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-5xTetO-pEF-H2B-Citrine-SV40
Plasmid#186966PurposeLentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.DepositorInsert5xTetO-pEF-H2B-Citrine-SV40
UseLentiviralExpressionMammalianPromoter5xTetO-pEF-1alphaAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN592 (FuGW-S(USF1)p-mKate2)
Plasmid#105204Purposesynthetic promoter USF1 drives mKate2 expressionDepositorInsertS(USF1)p-mKate2
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPE1
Plasmid#209981PurposeContains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmidsDepositorInsertE. coli shuttle part (pMB1_ampR_B0017)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPE2
Plasmid#209982PurposeContains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmidsDepositorInsertE. coli shuttle part (ampR_B0017)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPE3
Plasmid#209985PurposeContains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmidsDepositorInsertE. coli shuttle part (pMB1_cat)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP5
Plasmid#209967PurposeContains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmidsDepositorInsertPromoter (P_sppQ)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only