We narrowed to 7,669 results for: CCH

1. pFA6a-GST-kanMX6

   Plasmid

   #39293

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ033

   Plasmid

   #98404

   Purpose

   Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-GST-His3MX6

   Plasmid

   #41604

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_Puro

   Plasmid

   #167863

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFW5000

   Plasmid

   #224214

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB700 lenti gRNA Blasto_zhang2.0

   Plasmid

   #167912

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNeoXTR f0

   Plasmid

   #69157

   Purpose

   mouse targeting vector

   Depositor

   David Feldser

   Article

   Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

   Inserts

   DTA
   NeoR
   GFP
   AmpR
   

   Use

   Mouse Targeting

   Promoter

   Amp promoter, PGK, and none

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRG659_lexO-FseI_LexA-TF_LEU2MX

   Plasmid

   #154816

   Purpose

   Inducible expression of FseI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   FseI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIS3

    Plasmid

    #92173

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Expression

    Yeast

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPROEX Hsp104 (1-575)

    Plasmid

    #1318

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (1-575) (HSP104 Budding Yeast)
    

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa1-575 of Hsp104

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-NDP52

    Plasmid

    #171422

    Purpose

    For the purifcation of NDP52 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    NDP52 (CALCOCO2 Human)
    

    Expression

    Bacterial

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p415TEF cyto roGFP2-Grx1(yeast)

    Plasmid

    #65004

    Purpose

    Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1
    

    Tags

    roGFP2

    Expression

    Yeast

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH5

    Plasmid

    #52531

    Purpose

    expresses FLP recombinase in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Expression

    Insect

    Promoter

    ubiquitin

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Expression

    Yeast

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. LT2_14_GlyOx

    Plasmid

    #177711

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-QFGal4-SV40pA

    Plasmid

    #155126

    Purpose

    In vitro transcription of QFGal4 mRNA

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Expression in zebrafish

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

    Plasmid

    #79373

    Purpose

    Recruits VPR to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-VPR
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB700 lenti gRNA Puro_zhang2.0

    Plasmid

    #167919

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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