We narrowed to 9,405 results for: Coli

1. PLK1-cjun-FRET-PACT biosensor

   Plasmid

   #106907

   Purpose

   centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

   Depositor

   Heidi Hehnly

   Article

   Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

   Insert

   PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)
   

   Use

   Tags

   PACT domain

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-PLK1-mCherry

   Plasmid

   #127154

   Purpose

   Expresses mCherry PLK1 in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Colicino et al Mol Biol Cell. 2019 Jun 15;30(13):1598-1

   Insert

   PLK1-mCherry (PLK1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFLAG GLTP

   Plasmid

   #170740

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

   Insert

   GLTP (GLTP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. (PD-S608A)FLAG-Cep215-FL

   Plasmid

   #106905

   Purpose

   Expresses murine non-phosphorylatable Cep215 at S608

   Depositor

   Heidi Hehnly

   Article

   Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

   Insert

   CEP215 (Cdk5rap2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine-608 to Alanine

   Promoter

   CMV

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFLAG W96A-GLTP

   Plasmid

   #170741

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

   Insert

   GLTP (GLTP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   W96A

   Promoter

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFLAG CPTP

   Plasmid

   #170742

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

   Insert

   CPTP (CPTP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. (PM-S608E)FLAG-Cep215-FL

   Plasmid

   #106906

   Purpose

   Expresses murine phospho-mimetic CEP215 at S608

   Depositor

   Heidi Hehnly

   Article

   Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

   Insert

   CEP215 (Cdk5rap2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine-608 to Glutamic Acid

   Promoter

   CMV

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetO-Monbr-Sox-like

   Plasmid

   #231530

   Purpose

   Expresses Monsiga brevicolis full-length Sox-like in mammalian cells, for lentivirus generation

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox-like
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24a-ncOGT-FL

   Plasmid

   #190821

   Purpose

   For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E.coli

   Promoter

   T7

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBABE-SSeCKs T751A

    Plasmid

    #107142

    Purpose

    full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

    Insert

    SSeCKs T751A (Akap12 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed threonine 751 to alanine

    Promoter

    gag

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO FAPP2-GLTPH

    Plasmid

    #170739

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    PLEKHA8-GLTPH (PLEKHA8 Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO-FUW-NfiA

    Plasmid

    #64901

    Purpose

    Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    Nfia (Nfia Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET24a-ncOGT-TPR

    Plasmid

    #190822

    Purpose

    For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    C467X. Also codon optimized for expression in E.c…

    Promoter

    T7

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti HsATP13A2 WT (PAM) _ Hygro

    Plasmid

    #213697

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

    Depositor

    Veerle Baekelandt

    Article

    Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

    Insert

    human ATP13A2 WT PAM (ATP13A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT, D508N

    Promoter

    CMV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti HsATP13A2 P652_E653 del

    Plasmid

    #213700

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

    Depositor

    Veerle Baekelandt

    Article

    Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

    Insert

    human ATP13A2 P652_E653 del (ATP13A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT, D962N

    Promoter

    CMV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO GLTP

    Plasmid

    #170732

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32bplus_trxAChEwt

    Plasmid

    #83916

    Purpose

    Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

    Depositor

    Sarel Fleishman

    Article

    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

    Insert

    Short form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    

    Use

    Tags

    His, S-tag, and TRX

    Expression

    Bacterial

    Mutation

    codon optimised for E.coli expression

    Promoter

    T7 promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Use

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

    Plasmid

    #78507

    Purpose

    Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Gads SH3 SH2 SH3 domain (GRAP2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Promoter

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-28a(+)-FGF2-K128N

    Plasmid

    #120284

    Purpose

    Produce human recombinant FGF2 K128N in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDNA2.0 6H-TEV-KRAS

    Plasmid

    #111849

    Purpose

    Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid

    #111850

    Purpose

    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12V (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Valine

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti GW V5 Eco/Dam hum LaminB1

    Plasmid

    #182671

    Purpose

    Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.

    Depositor

    Bas van Steensel

    Article

    Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do

    Insert

    Lamin B1 (LMNB1 Human)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    HSP

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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