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We narrowed to 18,759 results for: nls

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Showing: 10441 - 10480 of 18759 results

  8. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid
    #208574
    Purpose
    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK/CMV and minP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208574-plasmid-map-sequence-id-413226

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  10. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

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  11. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  17. CCND1 targeting gRNA

    Plasmid
    #215153
    Purpose
    Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.
    Depositor
    Michele Pagano
    Publication
    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215153-plasmid-map-sequence-id-429224

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  20. 53BP1 N-terminal sgRNA

    Plasmid
    #207091
    Purpose
    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGGGAGCAGATGGACCCTAC
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207091-plasmid-map-sequence-id-427374

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  21. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216249
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus
    Depositor
    Elina Ikonen
    Publication
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    Use
    CRISPR; Endogenous tagging
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216249-plasmid-map-sequence-id-426851

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  22. pCLV3Cas-LT1

    Plasmid
    #210549
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Publication
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    Expression
    Plant
    Mutation
    2bp insertion after AAVS1 target sequence that di…
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210549-plasmid-map-sequence-id-426236

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  23. pMK33-V5-TurboID-GW

    Plasmid
    #196282
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196282-plasmid-map-sequence-id-384562

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  25. pCMV-enCas12a-A3A-Y130F

    Plasmid
    #193652
    Purpose
    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLb-enCas12a-hA3A-Y130F, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193652-plasmid-map-sequence-id-422135

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  26. pCMV-dCas12a-A3A-N57G

    Plasmid
    #193639
    Purpose
    Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-hA3A-N57G, EGFP
    Expression
    Mammalian
    Mutation
    hAPOBEC3A-N57G
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193639-plasmid-map-sequence-id-421922

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  27. pCMV-dCas12a-A3A-Y130F

    Plasmid
    #193640
    Purpose
    Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-hA3A-Y130F, EGFP
    Expression
    Mammalian
    Mutation
    hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193640-plasmid-map-sequence-id-421923

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  28. pCMV-dCas12a-A3A-Y132D

    Plasmid
    #193641
    Purpose
    Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-hA3A-Y132D
    Expression
    Mammalian
    Mutation
    hAPOBEC3A-Y132D
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193641-plasmid-map-sequence-id-421939

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Showing: 10441 - 10480 of 18759 results