We narrowed to 14,012 results for: Ung;

1. pM378

   Plasmid

   #173174

   Purpose

   Expresses C9-1 gRNA-12xMBS from hU6 promoter

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   C9-1 targeting gRNA-12xMBS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. UbG76V-eGFP-V5His_20aa

   Plasmid

   #23970

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_20aa
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Available Since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDHt/sk-CE

   Plasmid

   #92128

   Purpose

   Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei

   Depositor

   Zhihua Zhou

   Article

   Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

   Insert

   Cas9-eGFP
   

   Use

   CRISPR; Trichoderma reesei expression

   Tags

   EGFP

   Promoter

   Pcbh2

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p6618 MSCV-IP N-HAonly WHSC2

   Plasmid

   #35014

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   WHSC2 (NELFA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

5. PL-SIN-EOS-C(3+)-EGFP

   Plasmid

   #21318

   Purpose

   Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EOS-C(3+) promoter region

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad-mCherry-XL (R125K)

   Plasmid

   #188054

   Purpose

   Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   araBAD

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-H1.1

   Plasmid

   #32894

   Depositor

   Michael Hendzel

   Article

   Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

   Insert

   Histone H1.1 (H1-1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAX_CAGGS_CAS9_Hygro

   Plasmid

   #167859

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. #2.4 pre-G-SynapShot-Myc

    Plasmid

    #214722

    Purpose

    Expresses green #2.4 pre-SynapShot and Myc tag

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #2.4 pre-G-SynapShot
    

    Use

    AAV

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nanog5P reporter

    Plasmid

    #16337

    Depositor

    Austin Cooney

    Article

    Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

    Insert

    Nanog promoter (Nanog Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    Mouse Nanog promoter

    Available Since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330_human CDK1

    Plasmid

    #118597

    Purpose

    One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    guide RNA against human CDK1
    

    Use

    CRISPR

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC020 - LseCas13a from Listeria seeligeri

    Plasmid

    #91910

    Purpose

    Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LseCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ScPCK1

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHYX138

    Plasmid

    #202815

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ScADH2

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_112

    Plasmid

    #136475

    Purpose

    Lentiviral expression of AsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

    Plasmid

    #189807

    Purpose

    Retroviral delivery of Cas9

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

    Insert

    Cas9
    

    Use

    Retroviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-puro-miR-9 sponge

    Plasmid

    #25040

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    miR-9 sponge
    

    Use

    Retroviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC017 - LwCas13a guide expression backbone with tRNAval promoter

    Plasmid

    #91907

    Purpose

    Backbone for cloning LwCas13a guides under tRNAval promoter

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a Guide site
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGL3-VEGFR2-780

    Plasmid

    #21307

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-N-I-N-CAAX

    Plasmid

    #41686

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available Since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. NAX_CAGGS_CAS9_Blasto

    Plasmid

    #167856

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJH1371

    Plasmid

    #199631

    Purpose

    Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GPI
    

    Tags

    YFP

    Expression

    Worm

    Promoter

    Pregf-1

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. LV

    Plasmid

    #202004

    Purpose

    Empty lenti backbone

    Depositor

    Nikhil Joshi

    Article

    Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. phCMV-IE1

    Plasmid

    #118048

    Purpose

    GBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Cytomegalovirus enhancer and promoter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG_EGFP-GFE3

    Plasmid

    #79869

    Purpose

    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Promoter

    Beta-actin

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMH-EF1-GFP

    Plasmid

    #133018

    Purpose

    (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

    Plasmid

    #104119

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    CheRiff-CFP
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    human synapsin I

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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