Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 11,832 results for: NSI;

Clear keywords
Showing: 10481 - 10520 of 11832 results

  2. pEPQD1CB0108

    Plasmid
    #177041
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    Use
    Synthetic Biology
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177041-plasmid-map-sequence-id-349015

    Enlarge

  3. pEPQD1CB0817

    Plasmid
    #177039
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
    Use
    Synthetic Biology
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177039-plasmid-map-sequence-id-349012

    Enlarge

  4. pJT10R

    Plasmid
    #98307
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    TRPL3AcNES1>TRPL41B
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Available Since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98307-plasmid-map-sequence-id-190177

    Enlarge

  5. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid
    #127244
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    CamKIIa
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127244-plasmid-map-sequence-id-247231

    Enlarge

  6. ERroGFP_C147S_pCDNA3

    Plasmid
    #48630
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48630-plasmid-map-sequence-id-70378

    Enlarge

  7. pEPQD1CB0119

    Plasmid
    #177051
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    major latex protein-like (NmMLPL) from Nepeta mussinii
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177051-plasmid-map-sequence-id-349499

    Enlarge

  8. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Available Since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98306-plasmid-map-sequence-id-181809

    Enlarge

  10. ERrefGFP1_pCDNA3

    Plasmid
    #48635
    Purpose
    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERrefGFP1
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S,C204Q
    Available Since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48635-plasmid-map-sequence-id-70401

    Enlarge

  11. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

    Enlarge

  15. pCALNL_chr13_102010574-102010650

    Plasmid
    #81213
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81213-plasmid-map-sequence-id-163268

    Enlarge

  16. pCALNL_Ch12_62418577-62418652

    Plasmid
    #81212
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81212-plasmid-map-sequence-id-163264

    Enlarge

  17. mCherry_LD 0_CS M_tTA-BFP

    Plasmid
    #58874
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    58874-plasmid-map-sequence-id-94235

    Enlarge

  18. roGFP_iE_C204S_pQE30

    Plasmid
    #48632
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, c204S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48632-plasmid-map-sequence-id-70381

    Enlarge

  19. mCherry_LD 0_CS A_tTA-BFP

    Plasmid
    #58873
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58873-plasmid-map-sequence-id-94234

    Enlarge

  20. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

    Enlarge

  28. pTKI-Tyr

    Plasmid
    #247647
    Purpose
    Expresses GFP-SUMO-Tyr-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Insert
    GFP-SUMO-Tyr-mCherry N-degron proteolysis dual-fluorescence reporter
    Use
    Mycobacterial integrating
    Tags
    HA tag and Myc tag
    Expression
    Bacterial
    Promoter
    PTet
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247647-plasmid-map-sequence-id-494850

    Enlarge

  29. pTKI-Ser

    Plasmid
    #247648
    Purpose
    Expresses GFP-SUMO-Ser-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Insert
    GFP-SUMO-Ser-mCherry N-degron proteolysis dual-fluorescence reporter
    Use
    Mycobacterial integrating
    Tags
    HA tag and Myc tag
    Expression
    Bacterial
    Promoter
    PTet
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247648-plasmid-map-sequence-id-494849

    Enlarge

  34. pTARGEX-CHL

    Plasmid
    #242447
    Purpose
    Targets high levels of recombinant protein to chloroplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    Transit peptide from N. tabacum RbcS
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242447-plasmid-map-sequence-id-490473

    Enlarge

Showing: 10481 - 10520 of 11832 results