We narrowed to 20,388 results for: ACE;

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pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFusX1-31

Plasmid

#63249

Purpose

Encodes RVD positions 1-3 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-pfkB

Plasmid

#102284

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

pfkB gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pFusX4-31

Plasmid

#63440

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
5mCUC1 pBluescript

Plasmid

#12065

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Expression

Bacterial

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pAAV.hSynap.(cyto).iATPSnFR2.A95A.A119L.mCherry

Plasmid

#244920

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mCherry

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mCherry

Use

AAV

Mutation

A95A.A119L

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#244921

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mRuby3

Plasmid

#244913

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mRuby3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mRuby3

Plasmid

#244911

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mRuby3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mIRFP670nano3

Plasmid

#244917

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mIRFP670nano3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.mCherry

Plasmid

#244919

Purpose

iATPSnFR2.A95K variant with non-responsive mCherry

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mCherry

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mIRFP670nano3

Plasmid

#244916

Purpose

iATPSnFR2.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mIRFP670nano3

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mRuby3

Plasmid

#244912

Purpose

iATPSnFR2.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mRuby3

Use

AAV

Mutation

A95K

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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