We narrowed to 9,203 results for: sacs

1. pRS416 ATG5-E141D-HA-ATG12-ATG16

   Plasmid

   #166851

   Purpose

   Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

   Depositor

   Daniel Klionsky

   Article

   Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

   Insert

   ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)
   

   Use

   Tags

   HA-ATG12

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TT MEK5-DD-mRFP1

   Plasmid

   #47547

   Purpose

   Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

   Insert

   MEK5-DD (MAP2K5 Human)
   

   Use

   Entry vector

   Tags

   mRFP

   Expression

   Mutation

   S311D, T315D

   Promoter

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. flag-hKLF6 P149S  (1008)

   Plasmid

   #49496

   Purpose

   expresses human Flag tagged KLF6 with P149S mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   KFL6-P149S (KLF6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P149S

   Promoter

   CMV

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEN_TT 3xHA-MKK6-EE

   Plasmid

   #47544

   Purpose

   Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

   Insert

   3xHA-MKK6-EE (MAP2K6 Human)
   

   Use

   Entry vector

   Tags

   3XHA

   Expression

   Mutation

   S207E, T211E

   Promoter

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pETcon-pGAL1-Aga2-Amyloid β42-c_myc

   Plasmid

   #225223

   Purpose

   Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   Amyloid β42
   Aga2 (AGA2 Budding Yeast)
   

   Use

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-WzxE-TEV-His10

   Plasmid

   #226781

   Purpose

   Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

   Depositor

   James Naismith

   Article

   Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

   Insert

   wzxE
   

   Use

   Tags

   TEV cleavable 10xHistidine tag

   Expression

   Bacterial

   Mutation

   valine insertion in position 2 due to the cloning…

   Promoter

   araBAD

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM88

   Plasmid

   #226680

   Purpose

   Rpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6)

   Depositor

   Andreas Martin

   Article

   Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

   Inserts

   rpt1 (RPT1 Budding Yeast)
   Rpt2 (RPT2 Budding Yeast)
   Rpt3 (RPT3 Budding Yeast)
   Rpt4 (RPT4 Budding Yeast)
   Rpt5 (RPT5 Budding Yeast)
   Rpt6 (RPT6 Budding Yeast)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   I191TAG

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM89

   Plasmid

   #226681

   Purpose

   Rpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, Rpt6)

   Depositor

   Andreas Martin

   Article

   Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

   Inserts

   rpt1 (RPT1 Budding Yeast)
   Rpt2 (RPT2 Budding Yeast)
   Rpt3 (RPT3 Budding Yeast)
   Rpt4 (RPT4 Budding Yeast)
   Rpt5 (RPT5 Budding Yeast)
   Rpt6 (RPT6 Budding Yeast)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   Q49TAG

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CIRC#del

   Plasmid

   #186344

   Purpose

   Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

   Depositor

   Giovanni Blandino

   Article

   Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

   Insert

   VEGFA (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

    Plasmid

    #225490

    Purpose

    Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    Neuronal expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

    Plasmid

    #224779

    Purpose

    Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S131C / S562C

    Promoter

    CMV

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Plasmid

    #224755

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Plasmid

    #224756

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131A/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H6Gb1-CREB(1-280) T2C, C90A, T198C

    Plasmid

    #220235

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    T2C, C90A, T198C

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. H6Gb1-CREB(1-280) C90A, T172C, T259C

    Plasmid

    #220234

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    C90A, T172C, T259C

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIRHD-TetR-GAL80-Rho1-GAL4

    Plasmid

    #218274

    Purpose

    Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

    Depositor

    Christopher Howard

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    DHFR (P66L), Rho1 (C17R)

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TO-EGFR*-CUbo-GFP

    Plasmid

    #212815

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    8xHis and CUbo-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT-TO-EGFR*-CUbo-His6

    Plasmid

    #212819

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

    Plasmid

    #212822

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo(K63R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. kanMX6-ins1

    Plasmid

    #195038

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Coexpressed Free NES

    Plasmid

    #182482

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Coexpressed Free ClLIS1 (M)

    Plasmid

    #182484

    Purpose

    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS(M)
    LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNIA-CEN-FLAG-LANS1-Set2

    Plasmid

    #122002

    Purpose

    FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    histone methyltransferase SET2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. deltaVP1 + VP2C-GFP-MIOX

    Plasmid

    #166682

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MIOX only

    Plasmid

    #166681

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    MIOX (Miox Synthetic, Budding Yeast, Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. wtVP1 + VP2C-GFPdeg

    Plasmid

    #166678

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. deltaVP1 + VP2C-GFPdeg

    Plasmid

    #166677

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge