We narrowed to 9,939 results for: Uty

1. MSNA-c001

   Plasmid

   #175466

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG431

   Plasmid

   #165615

   Purpose

   Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Corrected homology relative to wild type SpCas9 (…

   Promoter

   TEF1

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ST-Tstem-GFP

   Plasmid

   #162501

   Purpose

   Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The Tac stem region is inserted within ST6Gal1

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Tac-STC

   Plasmid

   #162490

   Purpose

   Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its stem region, TM…

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)

   Plasmid

   #156412

   Purpose

   Gateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancer

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   murine Dll4 F2- ETS site B/ site #2, 8X
   

   Use

   Gateway cloning

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_PDPR_exon_deletion_2

   Plasmid

   #155066

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_SFRS7_exon_deletion_1

   Plasmid

   #155069

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLCHKO_SFRS7_exon_deletion_2

   Plasmid

   #155070

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLCHKO_MDM4_exon_deletion_1

    Plasmid

    #155073

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCHKO_MDM4_exon_deletion_2

    Plasmid

    #155074

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLCHKO_MDM4_exon_deletion_4

    Plasmid

    #155076

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCHKO_Ptbp_ex8_1

    Plasmid

    #155081

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKO_Ptbp_ex8_4

    Plasmid

    #155084

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCHKO_SFRS7_exon_deletion_4

    Plasmid

    #155072

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_Ptbp_ex8_2

    Plasmid

    #155082

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLCHKO_PDPR_exon_deletion_3

    Plasmid

    #155067

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKO_PDPR_exon_deletion_4

    Plasmid

    #155068

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

    Plasmid

    #105028

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

    Plasmid

    #105029

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge