We narrowed to 43,548 results for: cha;
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Plasmid#58731Purposeexpression of HA tagged mouse Alpha2 delta-2 in mamalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL
Plasmid#160288PurposeAllows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMX-PL1-WDR5
Plasmid#59970PurposeMammalian Expression of WDR5DepositorInsertWDR5 (WDR5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104095PurposeExpresses TREAT-siRNA reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_UBXN4_R3HDM1
Plasmid#205950PurposeExpress mEGFP-tagged fusion protein, UBXN4_R3HDM1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104094PurposeExpresses TREAT reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…ExpressionMammalianMutationPromoterTetCMVAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-HCoV-NL63-3CL
Plasmid#160286PurposeAllows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
APEX2-6xHp
Plasmid#214411PurposeLipid droplet localization of APEX2-6xHp for electron microscopyDepositorInsert6x Spastin aa 43-92 (APEX2 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX
Plasmid#169727PurposeMammalian expression construct of GFP tagged cDAXXDepositorInsertcDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-740PromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-Bat-CoV-HKU9-3CL
Plasmid#160284PurposeAllows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104096PurposeExpresses TREAT-ARE reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…ExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-WDR5
Plasmid#59969PurposeBacterial Expression GST-WDR5DepositorInsertWDR5 (WDR5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-DCTK75E
Plasmid#126423PurposeA kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertcalcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
UseTagsFLAGExpressionMammalianMutationchanged lysine 75 to glutamic acidPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-3xA
Plasmid#101041PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
UseTagsGFPExpressionMammalianMutationS1915A, S1916A, S1943APromoterCMVAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG IL13-CAR
Plasmid#157742Purposeknock IL13-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertChimeric antigen receptor targeting IL13
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-3CL
Plasmid#160280PurposeAllows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-WT(pL84)
Plasmid#124400PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.DepositorInsertGal4-TET1-878-911 wildtype (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCS2+ Zf ctnna1L344P
Plasmid#185407PurposeZebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMVDepositorInsertctnna1 (ctnna1 Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Leucine 344 to ProlinePromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 SOX17 g2
Plasmid#155188PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2DepositorInsertCas13d SOX17 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104140PurposeExpresses TREAT-5'TOP reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…ExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-N1-iChloC_CA
Plasmid#98171Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
UseTagsmCherryExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mitoX4-mCerulean
Plasmid#185807PurposeMitochondrial targetted (COX8) mCeruleanDepositorInsertmito-mCerulean
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ZC3H4_POLR2I
Plasmid#205956PurposeExpress mEGFP-tagged fusion protein, ZC3H4_POLR2I from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn-somAion-citrine
Plasmid#192570PurposeAAV-vector for optogenetic long-term silencing of neuronsDepositorInsertsomAion
UseAAVTagsCitrine - soma-targeting motif from Kv2.1 channelExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…Promoterhuman synapsinAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Phobos
Plasmid#98166Purposeblue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
UseTagsAID(71-114)ExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Aurora
Plasmid#98167Purposered-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Aurora gene
UseTagsmCherryExpressionMammalianMutationV59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-dDIO-lck-smMyc-4x6T
Plasmid#196421PurposeDre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Dre/rox; astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot8-D40AE42A_AH
Plasmid#148902PurposeMammalian Expression of HsNot8-D40AE42ADepositorInsertHsNot8-D40AE42A (CNOT8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Vimentin-7
Plasmid#57688PurposeLocalization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVimentin (VIM Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
139H2_HC
Plasmid#206201PurposeFor recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.DepositorInsertanti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
UseTags-AAAHHHHHHHHExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E/K534A hTFNG
Plasmid#70133PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobeDepositorInsertN6His K206e/K534A hTFNG (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid and Lys534 to a…PromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_RBM39_VMP1
Plasmid#205910PurposeExpress mEGFP-tagged fusion protein, RBM39_VMP1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa1 AS
Plasmid#69825PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
VP16-ER beta (short)
Plasmid#11353DepositorInsertER beta short (ESR2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-HCoV-NL63-3CL-C144A
Plasmid#160287PurposeAllows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC144APromoterEF1aAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-3CL-C145A
Plasmid#160281PurposeAllows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC145APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1
Plasmid#205444Purposehuman AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59DepositorInsertiCasp9-HLA-E SCT-hCD55
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d P2A eGFP-NLS
Plasmid#155182PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFPDepositorInsertCasRx
UseUnspecified; Piggybac transposonTagsNLS and P2A eGFP NLSExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only