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We narrowed to 18,759 results for: nls

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Showing: 10561 - 10600 of 18759 results

  3. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid
    #195315
    Purpose
    Dox inducible lentiviral Cas9 nickase
    Depositor
    Raj Chari
    Insert
    Cas9-nickase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A nickase
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    195315-plasmid-map-sequence-id-390525

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  4. pCRISPR Human CASP9 -2

    Plasmid
    #198420
    Purpose
    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein
    Depositor
    Hannah Rabinowich
    Publication
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CASP9 Specific gRNA (CASP9 Human)
    Use
    CRISPR
    Tags
    Cas9/Orange Fluorescent Protein Reporter
    Expression
    Mammalian
    Promoter
    U6; CMV
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198420-plasmid-map-sequence-id-390260

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  5. pCRISPR Human CASP9 -1

    Plasmid
    #198419
    Purpose
    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein
    Depositor
    Hannah Rabinowich
    Publication
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CASP9 Specific gRNA (CASP9 Human)
    Use
    CRISPR
    Tags
    Cas9/Orange Fluorescent Protein Reporter
    Expression
    Mammalian
    Promoter
    U6; CMV
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198419-plasmid-map-sequence-id-390148

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  8. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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  9. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  12. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197966
    Purpose
    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Publication
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-vCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Expression
    Plant
    Promoter
    CmYLCVp and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197966-plasmid-map-sequence-id-387890

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  13. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197979
    Purpose
    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Publication
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-vCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Expression
    Plant
    Promoter
    pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197979-plasmid-map-sequence-id-388023

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  15. pENeGFP PCNAL2 pc653

    Plasmid
    #167564
    Purpose
    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.
    Depositor
    Cristina Cardoso
    Publication
    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Tags
    GFP
    Expression
    Mammalian
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167564-plasmid-map-sequence-id-388295

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  24. pHR_PGK_SNIPR_CD8alpha variant 2, G318A

    Plasmid
    #188365
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Publication
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
    Use
    Lentiviral
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188365-plasmid-map-sequence-id-371170

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  31. AYBEv0.1

    Plasmid
    #193966
    Purpose
    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    Expression
    Mammalian
    Promoter
    hU6, EF1a, CBh
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193966-plasmid-map-sequence-id-384658

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  33. CABE-T2.19

    Plasmid
    #193265
    Purpose
    Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CABE-T2.19
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193265-plasmid-map-sequence-id-384183

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  35. pX459-sgRNA3-RPB1-N-term

    Plasmid
    #195112
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against first exon of RPB1 (N-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195112-plasmid-map-sequence-id-383882

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  36. pX459-sgRNA1-RPB1-N-term

    Plasmid
    #195110
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against first exon of RPB1 (N-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195110-plasmid-map-sequence-id-383880

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  37. pX459-sgRNA2-2-RPB1-C-term

    Plasmid
    #195109
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against last exon of RPB1 (C-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195109-plasmid-map-sequence-id-383879

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  38. pX459-sgRNA4-CTCF-3p-UTR

    Plasmid
    #195106
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against CTCF 3' UTR region
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195106-plasmid-map-sequence-id-383878

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  39. pX459-sgRNA3-CTCF-3p-UTR

    Plasmid
    #195105
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against CTCF 3' UTR region
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195105-plasmid-map-sequence-id-383877

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  40. pX459-sgRNA2-CTCF-prom

    Plasmid
    #195104
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against CTCF promoter
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195104-plasmid-map-sequence-id-383876

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Showing: 10561 - 10600 of 18759 results