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p5E 5X C120 c-fos min pro (JDW 1241)

Plasmid

#224544

Purpose

A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

5x-C120-c-Fos promoter

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXB1-WT

Plasmid

#215589

Purpose

For overexpression of Gal4-DBD HOXB1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXB1 (HOXB1 Human)

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_TRE3G_hRTN3E-Halo

Plasmid

#216252

Purpose

Tet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vector

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon-3 isoform e (RTN3 Human)

Use

Lentiviral

Tags

HaloTag

Promoter

TRE3G

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa_WT(N)

Plasmid

#215635

Purpose

For overexpression of Gal4-DBD CEBPa WT(N)

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa(N) (CEBPA Human)

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-AausFP1

Plasmid

#220605

Purpose

Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNK291

Plasmid

#219748

Purpose

DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - nnH3H_v2 - tSV40

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-lifeact-oROS-HT

Plasmid

#216420

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

lifeact-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroLITE

Plasmid

#215614

Purpose

For integration of CEBPa AroLITE mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroLITE (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NGN2-WT

Plasmid

#215607

Purpose

For overexpression of Gal4-DBD NGN2 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXC4-AroPERFECT

Plasmid

#215588

Purpose

For overexpression of Gal4-DBD HOXC4 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXC4 AroPERFECT (HOXC4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXC4-AroLITE_S

Plasmid

#215587

Purpose

For overexpression of Gal4-DBD HOXC4 AroLITE S

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXC4 AroLITE S (HOXC4 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NANOG-AroLITE_A

Plasmid

#215600

Purpose

For overexpression of Gal4-DBD NANOG AroLITE A

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NANOG AroLITE A (NANOG Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroPERFECT_IS15

Plasmid

#215615

Purpose

For integration of CEBPa AroPERFECT IS15 mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15 (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroPERFECT_IS10

Plasmid

#215616

Purpose

For integration of CEBPa AroPERFECT IS10 mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS10 (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_WT

Plasmid

#215613

Purpose

For integration of CEBPa WT mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCINeo-gp78 Mutant G2BR

Plasmid

#185355

Purpose

Assessing cellular requirement for G2BRgp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Mammalian

Mutation

aa 595-600 KRFLNK to AGAAGG

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 61-410

Plasmid

#185334

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 61-610 only

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

Plasmid

#201818

Purpose

A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

V5-ER-LBD

Expression

Mammalian

Promoter

CMV-IE

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-IRX5

Plasmid

#170693

Purpose

doxycycline-inducible overexpression of IRX5

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

IRX5 (IRX5 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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