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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Promoter

SFFV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV

Plasmid

#24828

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pmScarlet_C1

Plasmid

#85042

Purpose

Mammalian expression of mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-S2215Y

Plasmid

#69013

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2215Y

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX.TRE3G.AdProt

Plasmid

#131687

Purpose

A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus Protease

Use

Lentiviral

Promoter

TRE3GV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lifeact-7

Plasmid

#54586

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

Lifeact-mTagRFP-T

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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N370S GBA pcDNA3.1

Plasmid

#188583

Purpose

Ectopic expression N370S GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Mutation

N370S

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCYC1m_yeGFP

Plasmid

#64389

Purpose

encodes yeast enhanced GFP under control of minimal pCYC1 promoter

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced GFP

Expression

Yeast

Promoter

pCYC1

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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His-GST-TEV-BRD4(42-459) BD1-BD2

Plasmid

#233887

Purpose

pGex for protein overexpression of human BRD4

Depositor

Alessio Ciulli

Article

Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

Insert

BRD4 (BRD4 Human)

Tags

His-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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