We narrowed to 22,195 results for: his

1. pET151-GFP-PP-RPN2 (797-953)

   Plasmid

   #74228

   Purpose

   [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 mCherry Gamma 3

   Plasmid

   #65234

   Purpose

   To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

   Depositor

   Narasimhan Gautam

   Article

   Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

   Inserts

   mcherry
   Gng3 (Gng3 Mouse)
   

   Use

   Tags

   6-His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pETDuet-msGC-NT2

   Plasmid

   #74763

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Hu et al J Biol Chem. 2008 Jul 25;283(30):20968-7

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 49-471 of the alfa subunit

   Promoter

   T7

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET151-hUCH37 (ISF1)(M148D/F149D)

   Plasmid

   #71388

   Purpose

   Expression of hUCH37 (M148D/F149D) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
   

   Use

   Tags

   HIS-TEV sequence

   Expression

   Bacterial

   Mutation

   changed methionine 148 to aspartate, phenylalanin…

   Promoter

   T7

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCellFree_G03 BMPR1A

   Plasmid

   #67105

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BMPR1A (BMPR1A Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCellFree_G03 EHD2

   Plasmid

   #67107

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EHD2 (EHD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET151-hUCH37 DM

   Plasmid

   #61931

   Purpose

   Expression of hUCH37 (ISF1) M269E/N291D in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) M269E/N291D (UCHL5 Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Changed methionine 269 to glutamate and asparagin…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET151-hUCH37 (ISF1)(M148A/F149A)

   Plasmid

   #61933

   Purpose

   Expression of hUCH37 (M148A/F149A) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) (UCHL5 Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Methionine 148 and Phenylalanine 149 were mutated…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET30Xa-SBL1(160E)

   Plasmid

   #66846

   Purpose

   Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   S160E

   Promoter

   T7lac

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT mir-1-1 reporter hsa-mir-128-1

    Plasmid

    #46743

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-128-1 (MIR128-1 Human)
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSETC PITPbeta N89F

    Plasmid

    #58279

    Purpose

    bacterial expression of PITP beta N89F mutant

    Depositor

    Shamshad Cockcroft

    Article

    Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N89F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pRSETC-PITPbeta (splice variant 1) K61A

    Plasmid

    #58271

    Purpose

    bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET-GST-TNRC6A-(935-984)-NES-mut

    Plasmid

    #42051

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    contain aa935-984, F951A, F955A, I958A, F960A

    Promoter

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQTEV-DKFZP566J2046

    Plasmid

    #34818

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    fumarylacetoacetate hydrolase domain containing 1 (FAHD1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQTEV-PTD012

    Plasmid

    #34797

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PTD012 (C11orf54 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IFI16-(192-393)-HinA-E222A-E272A

    Plasmid

    #35060

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; E222A and E272A…

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

17. IFI16-(192-393)-HinA-Y218A-Y267A

    Plasmid

    #35059

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; Y218A and Y267A…

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

18. IFI16-(192-393)-HinA-E381A

    Plasmid

    #35058

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; E381A mutation

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

19. IFI16-(192-393)-HinA-E272A

    Plasmid

    #35057

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; E272A mutation

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

20. BRCA1-(936-1113)

    Plasmid

    #35075

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains aa 936-1113

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

21. BRCA1-(1113-1316)

    Plasmid

    #35077

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains aa 1113-1316

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

22. BRCA1-(894-1057)

    Plasmid

    #35074

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains aa 894-1057

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

23. IFI16-(192-393)-HinA-E222A

    Plasmid

    #35056

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; E222A mutation

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

24. IFI16-(192-393)-HinA-T220A

    Plasmid

    #35055

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; T220A mutation

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

25. IFI16-(192-393)-HinA-Y218A

    Plasmid

    #35054

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; Y218A mutation

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

26. Tpa1(21-644)

    Plasmid

    #23140

    Depositor

    SeWon Suh

    Article

    Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

    Insert

    Tpa1 (TPA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-20

    Promoter

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

27. BacPAK Flag-NFRKB 1-101

    Plasmid

    #19792

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    only contains amino acids 1-101

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pET28a-MED11

    Plasmid

    #15414

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED11 (Med11 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

29. DCT.VV.Orf203

    Plasmid

    #12891

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf203
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

30. DCT.VV.Orf215

    Plasmid

    #12903

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf215
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

31. DCT.VV.Orf216

    Plasmid

    #12904

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf216
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

32. DCT.VV.Orf218

    Plasmid

    #12906

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf218
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

33. DCT.VV.Orf220

    Plasmid

    #12908

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf220
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

34. DCT.VV.Orf227

    Plasmid

    #12915

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf227
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

35. DCT.VV.Orf228

    Plasmid

    #12916

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf228
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

36. DCT.VV.Orf233

    Plasmid

    #12921

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf233
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

37. DCT.VV.Orf235

    Plasmid

    #12923

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf235
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

38. DCT.VV.Orf236

    Plasmid

    #12924

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf236
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

39. DCT.VV.Orf237

    Plasmid

    #12925

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf237
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

40. DCT.VV.Orf238

    Plasmid

    #12926

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf238
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only