We narrowed to 9,801 results for: pET
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6xHis-MBP-huBoCas12a
Plasmid#174673PurposeExpresses 6xHis-MBP tagged BoCas12aDepositorArticleInserthuBoCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
Tags6XHisExpressionBacterialMutationnonePromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialPromoterT7Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
KSP_K560GFP
Plasmid#129770PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKSP (kif11.L Frog)
TagsGFP and His6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…Available SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180CBM3
Plasmid#85090PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450 BM3
TagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3PromoterT7 promoterAvailable SinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK1_HUMAN_D0
Plasmid#79728PurposeThis plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK24_HUMAN_D0
Plasmid#79742PurposeThis plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1E_HUMAN_D0
Plasmid#79724PurposeThis plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MK13_HUMAN_D0
Plasmid#79693PurposeThis plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK13
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCri-1b
Plasmid#61327PurposeProtein expression.DepositorInsertGreen fluorescent protein
TagsHis-tag and MBP-tagExpressionBacterialAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-4a
Plasmid#61314PurposeProtein expression.DepositorInsertGreen fluorescent protein
TagsHis-tag and TRX-tagExpressionBacterialAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK_kanR_proJK1_PAD
Plasmid#60630PurposeConstitutively expresses PAD (phenolic acid decarboxylase)DepositorAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCoofy36
Plasmid#43994DepositorTypeEmpty backboneTagsHis10 and S-OneStrepExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only
