161,547 results
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pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
UseTags8xHIS and BPNLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationV93QPromoterT7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
COM-epegRNA-Dnmt1
Plasmid#211376PurposeDnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP productionDepositorInsertCOM-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
piRFP670-N1
Plasmid#45457DepositorHas ServiceCloning Grade DNAInsertiRFP670
UseTagsExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-Vc
Plasmid#183157PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HTR1D-Tango
Plasmid#66406PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHTR1D (HTR1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM
Plasmid#206206PurposeConstitutive expression of BsmAIM methyltransferaseDepositorInsertBsamAIM
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP 695
Plasmid#30137DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695PromoterAvailable sinceAug. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1
Plasmid#122147PurposeSelf-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.DepositorInsertFTH1 (FTH1 Human)
UseLentiviralTagsNLS-iLID-GFPExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-E
Plasmid#78852PurposeIntroduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsCas9-P2A-PuroExpressionMammalianMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-GFP1-10
Plasmid#80409PurposeExpresses GFP1-10 in mammalian cellsDepositorInsertGFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGK mScarlet-Synaptophysin
Plasmid#206145PurposePGK mScarlet-SynaptophysinDepositorInsertsynaptophysin (Syp Rat)
UseTagsmScarletExpressionMammalianMutationPromoterPGKAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-mt4xi-GCaMP6f
Plasmid#192075PurposeFluorescent reporter for mitochondrial calciumDepositorInsert4 repeats of improved COX8 signal peptide plus GCaMP6f
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu …PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR_Cas9_sp1
Plasmid#154851PurposeExpresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genomeDepositorInsertsSpcas9 gene
sgRNA scaffold
UseCRISPRTags6xHisExpressionMutationCodon harmonized variant for expression in Rhodob…PromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA_ASX
Plasmid#98888PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterCaMV35S (enhanced)Available sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N3-EPAC1
Plasmid#113110PurposeHuman EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).DepositorInsertRapGEF3 (RAPGEF3 Human)
UseTagsEYFPExpressionMammalianMutationPromoterPCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-PASS
Plasmid#193970PurposeMonitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)DepositorInsertPA biosenor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX1
Plasmid#112702PurposeExpression of NFIX1 protein and GFPDepositorInsertNuclear Factor I X1 (Nfix Mouse)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
