We narrowed to 9,184 results for: Pol;

1. pFB1_hMSH2

   Plasmid

   #129423

   Purpose

   Expression human MSH2 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MSH2 (MSH2 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPERretro-Sirt7 shRNA2

   Plasmid

   #53145

   Purpose

   shRNAi vector for Sirt7

   Depositor

   Katrin Chua

   Article

   Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

   Insert

   Sirt7
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   H1 RNA polymerase III

   Available Since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSUPERretro-Sirt7 shRNA1

   Plasmid

   #53144

   Purpose

   shRNAi vector for Sirt7

   Depositor

   Katrin Chua

   Article

   Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

   Insert

   Sirt7
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   H1 RNA polymerase III

   Available Since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. (SUMO)10

   Plasmid

   #126948

   Purpose

   Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH1002-Tier2(pUC ori)

   Plasmid

   #169600

   Purpose

   Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK313

   Plasmid

   #59570

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTK317

   Plasmid

   #59571

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(16) 2xK-turn Kozak EGFP attB2 AscI
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0040-LwaCas13a crRNA backbone

   Plasmid

   #103851

   Purpose

   Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-LwaCas13aDR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid

    #99689

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSmart-EF1a-Nla-cas3

    Plasmid

    #178881

    Purpose

    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas3c-bGH polyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGF_RT_FAST_RFP

    Plasmid

    #136979

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Article

    Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

    Insert

    seed coat marker RFP
    

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a(+)::NL

    Plasmid

    #141289

    Purpose

    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP207-AAV-CMV-MCS-pA

    Plasmid

    #78318

    Purpose

    AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-dSa-VPR mini.-1X snRP1

    Plasmid

    #99687

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP813

    Plasmid

    #113308

    Purpose

    human Mre11 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

    Insert

    Mre11 (MRE11 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB1_hPMS2

    Plasmid

    #129425

    Purpose

    Expression human PMS2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human PMS2 (PMS2 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 7MEQ

    Plasmid

    #176412

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    

    Use

    Baculovirus expresssion

    Tags

    8 x His Tag and Honeybee melittin signal

    Mutation

    etmprss2_S251D/R252D/Q253D/S254D/R255K

    Promoter

    Polyhedrin

    Available Since

    Oct. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pFB1_hMLH1

    Plasmid

    #129426

    Purpose

    Expression human MLH1 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MLH1 (MLH1 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2_hMGFP-60A

    Plasmid

    #200393

    Purpose

    A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    monster GFP
    

    Expression

    Mammalian

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. Arp2/3_pBIG2abcd

    Plasmid

    #173683

    Purpose

    Insect expression of the 7-subunit Human Arp2/3 complex

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Inserts

    Arp2
    Arp3
    ArpC2
    ArpC3-FLAG
    ArpC4
    ArpC5
    ArpC1
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pTK194

    Plasmid

    #59563

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak mKate opal attB2 AscI
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. TAF1

    Plasmid

    #39117

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1 (TAF1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

26. ATM-Halo HRD

    Plasmid

    #207082

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    None

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCoofy44

    Plasmid

    #55185

    Purpose

    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

    Expression

    Bacterial

    Promoter

    pPH (Polyhedrin promoter)

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV-Tag1-NS5A

    Plasmid

    #17646

    Depositor

    Xin Wang

    Article

    Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

    Insert

    HCV NS5A (POLY Hepatitis C virus)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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29. YFP-CAAX

    Plasmid

    #223558

    Purpose

    Plasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box

    Depositor

    Mikel Garcia-Marcos

    Article

    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

    Insert

    YFP(Venus) - KRas4b
    

    Expression

    Mammalian

    Available Since

    March 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pCMV-Tag1-NS3

    Plasmid

    #17645

    Depositor

    Xin Wang

    Article

    Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

    Insert

    HCV NS3 (POLY Hepatitis C virus)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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