163,445 results

1. 2X_pX458_pSpCas9(BB)-2A-GFP

   Plasmid

   #172221

   Purpose

   Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2FA CO Tol2 TPase

   Plasmid

   #133032

   Purpose

   Expression vector for production of codon optimized Tol2 transposase

   Depositor

   Charles Nathan Hancock

   Article

   Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

   Insert

   Codon optimized Tol2 Transposase
   

   Use

   In vitro expression

   Tags

   Expression

   Mutation

   Codon Optimized

   Promoter

   Available since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX_305-N-dTAG

   Plasmid

   #91797

   Purpose

   Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Flpe

   Plasmid

   #13787

   Purpose

   Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Flpe
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV Puro

   Plasmid

   #68469

   Purpose

   Retroviral plasmid with multiple cloning site and puromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Puromycin Resistance
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-FLAG-SF2

   Plasmid

   #99021

   Purpose

   Mammalian expression of SF2

   Depositor

   Honglin Chen

   Article

   Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

   Insert

   SF2 (SRSF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMS-II

   Plasmid

   #204765

   Purpose

   GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-m6Alinker-DHFR
   

   Use

   Tags

   Expression

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSSRB069_pAC8-hsDDB1dB

   Plasmid

   #218790

   Purpose

   Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Deleted amino acids 396-705 and replaced with GNG…

   Promoter

   polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

    Plasmid

    #100848

    Purpose

    AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1 and AAV9

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyTag003-MBP

    Plasmid

    #133450

    Purpose

    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GFP

    Plasmid

    #11153

    Purpose

    Mammalian expression vector for expression of GFP (CMV promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    CMV promoter-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN837

    Plasmid

    #184825

    Purpose

    Expresses human KIF5A in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (KIF5A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-LAMP1-HaloTag

    Plasmid

    #164209

    Purpose

    Expresses HaloTag tagged LAMP1 under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. EZ-Tet-pLKO-Puro

    Plasmid

    #85966

    Purpose

    Tet/Dox inducible shRNA lentivirus with puromycin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1 / TetO

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-D50 lamin A

    Plasmid

    #17653

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

    Insert

    D50 lamin A (LMNA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta 50 (50 aa internal deletion in globular tai…

    Promoter

    CMV

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV-AmphoEnv

    Plasmid

    #15799

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    4070A Amphotropic Murine leukemia virus envelope gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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