Skip to main content
Addgene
Showing: 781 - 810 of 5864 results

  4. pcDNA3.1(-)-flag-UBR2

    Plasmid
    #50573
    Purpose
    Expresses FLAG-tagged mouse UBR2 in mammalian cells.
    Depositor
    Alexander Varshavsky
    Insert
    UBR2 (Ubr2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pcDNA3.1-MS2-HA-HshnRNPK_Z

    Plasmid
    #148124
    Purpose
    Mammalian Expression of HshnRNPK
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPK (HNRNPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148124-plasmid-map-sequence-id-290571

    Enlarge

  6. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα11-LgB97 (GNA11 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

    Enlarge

  10. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα13-LgB106 (GNA13 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

    Enlarge

  13. pcDNA3.1-mPCSK3 H194L

    Plasmid
    #122689
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Histidine 194 to Leucine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122689-plasmid-map-sequence-id-244742

    Enlarge

  14. pcDNA3.1-ccdB-Renilla

    Plasmid
    #87065
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87065-plasmid-map-sequence-id-173589

    Enlarge

  15. pcDNA3.1-mPCSK3 S368A

    Plasmid
    #122680
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Serine 368 to Alanine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122680-plasmid-map-sequence-id-244743

    Enlarge

  20. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

    Plasmid
    #148118
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148118-plasmid-map-sequence-id-290565

    Enlarge

  21. pcDNA3.1_C-6His-Stx17

    Plasmid
    #112259
    Purpose
    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)
    Depositor
    Madhulika Tripathi
    Insert
    syntaxin 17 (Stx17 Mouse)
    Use
    Tags
    6His Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112259-plasmid-map-sequence-id-217085

    Enlarge

  22. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

    Enlarge

Showing: 781 - 810 of 5864 results