Addgene

We narrowed to 4,054 results for: abo

Showing: 1041 - 1080 of 4054 results

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pYES2.1-cerMET6

Plasmid

#107438

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET6 (MET6 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKJ237

Plasmid

#212320

Purpose

Express KnFnuc

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ226

Plasmid

#212316

Purpose

Express DpFnuc with pureexpress

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP

Plasmid

#214363

Purpose

Expression of the Blue Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET10

Plasmid

#107440

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET10 (MET10 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-Cer.zf1

Plasmid

#61389

Purpose

mRNA synthesis of cerulean codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cer.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET16

Plasmid

#107442

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET16 (MET16 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

PEP-carboxykinase promoter

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8380

Plasmid

#126914

Purpose

Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-Per

Plasmid

#33157

Depositor

Michael Rosbash

Article

Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

Insert

Per (per Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pRSET FLII12Pglu-600u

Plasmid

#13563

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Y12S-ECFP-D13R

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A ECFP insertion in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR-QK1

Plasmid

#16183

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Quaking (QKI Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pBbE8k-PP3359-6xHis

Plasmid

#83067

Purpose

Expresses 6xHis tagged PP3359 under pBAD regulation

Depositor

Matthew Chang

Article

Lo et al Cell Syst. 2016 Aug;3(2):133-43. doi: 10

Insert

PP3359

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-170nDelta13

Plasmid

#13562

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

WT binding protein (no F16A)

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-3.2mDelta13

Plasmid

#13560

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, S112A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSLeu2d-BISopt

Plasmid

#35524

Depositor

Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

P GAL 1

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET FLII275Pglu-4.6m

Plasmid

#13565

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A G275S-ECFP-K276R

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

-

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET17

Plasmid

#107443

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET17 (MET17 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB8383

Plasmid

#126917

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

tHMG1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-2uDelta13

Plasmid

#13561

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F D152A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, D152A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mutation

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pYES2.1-pomMet17

Plasmid

#107453

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met17 (met17 Fission Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pML2003

Plasmid

#193255

Purpose

Propionate sensor expressing eYFP reporter

Depositor

Lauren Andrews

Article

Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

Inserts

prpR
eYFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Pprp and Synthetic promoter P6

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8622

Plasmid

#126912

Purpose

Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5k-EPL95

Plasmid

#45434

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

AcrB/AcrD/AcrFa, ABO_0964

Use

Synthetic Biology

Tags

His

Expression

Mutation

Promoter

LacUV5

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMars1CyeGFP

Plasmid

#75222

Purpose

Mammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusion

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB4C5_pBen-BenR

Plasmid

#128127

Purpose

Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

BenR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
POT5-TEF2p-ycrtYB-ADH1t

Plasmid

#65359

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtYB-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POT2-TEF2p-ycrtE-ADH1t

Plasmid

#65353

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtE-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD398

Plasmid

#91832

Purpose

AID NT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EZ-L498

Plasmid

#131771

Purpose

PPGK1_FUSN_Citrine _PixE_TCYC1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PPGK1_FUSN_Citrine _PixE_TCYC1

Use

Integration into his3 locus

Tags

Expression

Yeast

Mutation

Promoter

PGK

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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