We narrowed to 1,483 results for: MBP

1. bdSUMO-5.3-GFP-HiBit

   Plasmid

   #171014

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-5.3-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EcNusGKOW

   Plasmid

   #113124

   Purpose

   Expresses C-terminus of E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-124

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EcRfaHKOW

   Plasmid

   #113125

   Purpose

   Expresses C-terminus of E. coli RfaH

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJB03

   Plasmid

   #111961

   Purpose

   Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Inserts

   mEGFP
   eDHFR
   

   Use

   Tags

   DHFR

   Expression

   Bacterial

   Mutation

   eDHFR;residues 2-159 and enhanced GFP with monome…

   Promoter

   Tac

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ICMAB - pET22b T7 EncFtn Mms6

   Plasmid

   #225538

   Purpose

   Construction of intracellular magnetite accumulating bacterial systems.

   Depositor

   Urartu Seker

   Article

   Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

   Inserts

   Iron binding protein
   Ferroxidase
   Material binding peptite
   Iron transporter protein
   

   Use

   Tags

   no

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMTTH+H1.4

   Plasmid

   #157796

   Purpose

   Expression of human linker histone H1.4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4 (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJ4M_hnRNPA2_FL_WT

   Plasmid

   #139109

   Purpose

   Express FL hnRNPA2 with a C-terminal MBP tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

   Plasmid

   #101209

   Purpose

   Bacterial expression plasmid for SpCas9-HF1 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Plasmid

   #101215

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A and R1060A

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hCD12

    Plasmid

    #225541

    Purpose

    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. B1 Integrin-GFP

    Plasmid

    #186138

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    MBP and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

    Plasmid

    #101205

    Purpose

    Bacterial expression plasmid for SpCas9 REC3 domain

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K506–Q712
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K506–Q712

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ4M_hnRNPA2_FL_P298L

    Plasmid

    #139111

    Purpose

    Express FL hnRNPA2 P298L with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P298L

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

    Plasmid

    #101213

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K855A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. polySUMO1

    Plasmid

    #221066

    Purpose

    Expression of polySUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PTE_25

    Plasmid

    #111638

    Purpose

    Funclib design PTE_25

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_25
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PTE_26

    Plasmid

    #111639

    Purpose

    Funclib design PTE_26

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_26
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PTE_27

    Plasmid

    #111640

    Purpose

    Funclib design PTE_27

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_27
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PTE_28

    Plasmid

    #111641

    Purpose

    Funclib design PTE_28

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_28
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PTE_36

    Plasmid

    #111642

    Purpose

    Funclib design PTE_36

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_36
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PTE_27.14

    Plasmid

    #111643

    Purpose

    Funclib design PTE_27.14

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_27.14
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. dPTE2

    Plasmid

    #111634

    Purpose

    PROSS stabilized PTE S5

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    dPTE2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PTE_5

    Plasmid

    #111635

    Purpose

    Funclib design PTE_5

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_5
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PTE_13

    Plasmid

    #111636

    Purpose

    Funclib design PTE_13

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_13
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PTE_24

    Plasmid

    #111637

    Purpose

    Funclib design PTE_24

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_24
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant

    Plasmid

    #101206

    Purpose

    Bacterial expression plasmid for SpCas9 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMTTH+mEGFP-H1.4

    Plasmid

    #157799

    Purpose

    Expression of mEGFP and human linker histone H1.4 fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-H1.4 (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMTTH+H1.4ΔCTD

    Plasmid

    #157797

    Purpose

    Expression of human linker histone H1.4 without c-terminal tail

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4ΔCTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid

    #101236

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K918A/V922A/R925A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K918A, V922A, R925A and Q926A

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMAL c2x Tral FL

    Plasmid

    #113012

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral WT

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid

    #101235

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    F491A, M495A, T496A, N497A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid

    #101234

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    T657A, G658A, W659A, R661A and Q926A

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hSRAP.HMG6.wt.Dlink.a1-236

    Plasmid

    #49813

    Purpose

    expression vector for MBP-SRAP(1-236) fusion

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein (SRA1 Human)
    

    Use

    Tags

    E. coli Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. poly H35S SUMO1

    Plasmid

    #221071

    Purpose

    Expression of poly H35S SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    H35S

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. poly E67R SUMO1

    Plasmid

    #221072

    Purpose

    Expression of poly E67R SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    E67R

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. poly H35K SUMO1

    Plasmid

    #221070

    Purpose

    Expression of poly H35K SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    H35K

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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43. poly H35R SUMO1

    Plasmid

    #221069

    Purpose

    Expression of poly H35R SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    H35R

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMAL-c5e-Rubicon RH

    Plasmid

    #216706

    Purpose

    Bacterial protein expression of Rubicon RH domain

    Depositor

    James Hurley

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid

    #179526

    Purpose

    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 KES(1107-1109)GG
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    replaced KES (residues 1107-1109) with GG

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid

    #179527

    Purpose

    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 K233A/K234A/K253A/K263A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed lysines 233, 234, 253, and 263 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. EcRfaHKOW-L1L2NusG

    Plasmid

    #113126

    Purpose

    Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107, replaced L1 and L2 loops …

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMTTH+mEGFP-H1.4ΔCTD

    Plasmid

    #157800

    Purpose

    Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-H1.4ΔCTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMTTH+LANA-H1.4CTD

    Plasmid

    #157798

    Purpose

    Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    LANA-H1.4CTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHTSHP-ShhN-Cys

    Plasmid

    #121136

    Purpose

    Expresses ShhN with a C-terminal cysteine residues in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, and MBP

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-191, with an addition…

    Promoter

    tac

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge