We narrowed to 1,772 results for: rpe

1. pLJC6-Flag CASTOR1

   Plasmid

   #84496

   Purpose

   Lentiviral expression of CASTOR1

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 (GATSL3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   sestrin2

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA4/TO-UL87-2xStrep

   Plasmid

   #138434

   Purpose

   Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   UL87 (UL87 HCMV (HHV-5))
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQDKN0761

   Plasmid

   #185630

   Purpose

   Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD1CB0107

   Plasmid

   #177040

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPQD1CB0202

   Plasmid

   #185626

   Purpose

   MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMAL-c2X-ORF24-NTD

   Plasmid

   #138465

   Purpose

   Expresses MBP-tagged ORF24-NTD in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Amino acids 1-201

   Promoter

   tac

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX4T1-ORF24-NTD

   Plasmid

   #138464

   Purpose

   Expresses GST-tagged ORF24-NTD in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 1-201

   Promoter

   tac

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4/TO-ORF24-3xFLAG

   Plasmid

   #138423

   Purpose

   Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSHDY*in_PpetE:BCD2:mVenus

   Plasmid

   #133970

   Purpose

   Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

   Depositor

   Pia Lindberg

   Article

   Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

   Insert

   mVenus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   inserted gene is codon optimized for Synechocyst…

   Promoter

   PpetE:BCD2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p6595 MSCV-IP-N-HA-GPRC5B

    Plasmid

    #34894

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    GPRC5B (GPRC5B Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLJC6-Flag CASTOR1 I280A

    Plasmid

    #84497

    Purpose

    Lentiviral expression of CASTOR2 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    sestrin2

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

12. p6H-SUMO3-ORF24-NTD-Strep

    Plasmid

    #138467

    Purpose

    Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    SUMO3 and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Amino acids 2-201

    Promoter

    T7

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET51b-His-TEV-SNAP-tag_cx

    Plasmid

    #167273

    Purpose

    Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    P179R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

    Plasmid

    #119316

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 481-852

    Promoter

    CMV

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-HA CASTOR2 ACT2

    Plasmid

    #84494

    Purpose

    overexpression of CASTOR2 ACT2 domain (aa 171-end)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 ACT2 (GATSL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    171-last amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-Flag CASTOR2 ACT1

    Plasmid

    #84493

    Purpose

    overexpression of CASTOR2 ACT1 domain (aa 1-171)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 ACT1 (GATSL2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-171 amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-HA CASTOR1 ACT2

    Plasmid

    #84492

    Purpose

    overexpression of CASTOR1 ACT2 domain (aa 169-end)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 ACT2 (GATSL3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    169-last amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-Flag CASTOR1 ACT1

    Plasmid

    #84491

    Purpose

    overexpression of CASTOR1 ACT1 domain (aa 1-169)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 ACT1 (GATSL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-169 amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. beta4-nAChR shRNA (Beta-4.3)

    Plasmid

    #86650

    Purpose

    expression of shRNA targeting CHRNB4

    Depositor

    Jaime Modiano

    Article

    Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

    Insert

    CHRNB4 (beta4-nAChR) (CHRNB4 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-FLAG-Kaptin

    Plasmid

    #100511

    Purpose

    Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    KPTN (KPTN Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACE SOX (ORF37) D221N E244Q

    Plasmid

    #192053

    Purpose

    Expresses a 2X catalytic mutant of (ORF37) D221N E244Q

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (D221N E244Q)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    2X catalytic mutant D221N E244Q

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUN378 - pTRV2-CrGLK

    Plasmid

    #203888

    Purpose

    Viral silencing plasmid targeting the CrGLK gene from C. roseus.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK fragment
    

    Use

    RNAi

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

    Plasmid

    #199447

    Purpose

    Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    dSaCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIPKAH

    Plasmid

    #185869

    Purpose

    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mTln1-F2-P229S-pET151

    Plasmid

    #177871

    Purpose

    Expresses mouse Talin 1 F2-P229S domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 P229S mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTln1-F2-P229L-pET151

    Plasmid

    #177876

    Purpose

    Expresses mouse Talin 1 F2-P229L domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 P229L mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEPQDKN0760

    Plasmid

    #185629

    Purpose

    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEPQD0KN0751

    Plasmid

    #185628

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOME0233M

    Plasmid

    #178415

    Purpose

    Reverse compl seq of pOME0134 flanked by Kpn and EcoRI for insertion into pcDNA3.1-

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    Reverse complement of pOME0134 upstream MIR-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOME0232M

    Plasmid

    #178414

    Purpose

    REVERSE complement seqeunce KSHV Downstream Mir-SYN RNA IN PCDNA3.1-

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV Reverse complement of downstream MIR-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEPQD1CB0869

    Plasmid

    #177086

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEPQD1CB0867

    Plasmid

    #177085

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEPQD1CB0806

    Plasmid

    #177080

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEPQD1CB0804

    Plasmid

    #177079

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEPQD1CB0798

    Plasmid

    #177077

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPQD1CB0792

    Plasmid

    #177076

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    major latex protein-like (NmMLPL) from Nepeta mussinii
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEPQD1CB0786

    Plasmid

    #177071

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEPQD1CB0785

    Plasmid

    #177070

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEPQD1CB0768

    Plasmid

    #177058

    Purpose

    MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEPQD1CB0124

    Plasmid

    #177056

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEPQD1CB0120

    Plasmid

    #177052

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    major latex protein-like (NmMLPL) from Nepeta mussinii
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEPQD1CB0861

    Plasmid

    #177078

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEPQD1CB0796

    Plasmid

    #177074

    Purpose

    MoClo Level 1, position 4, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEPQD0CM0065

    Plasmid

    #177019

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    transit peptide removed, amplified from cDNA, con…

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET51b-His-TEV-SNAP-tag_dead_mt

    Plasmid

    #167270

    Purpose

    Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid

    #162654

    Purpose

    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA4/TO-ORF24 1-201-2xStrep

    Plasmid

    #138442

    Purpose

    Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-201

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge