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Plasmid#82377PurposedCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBL119-2
Plasmid#104336PurposeExpresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.DepositorInsertThiosulfate response regulator
UseTagsExpressionBacterialMutationPromoterPLTetO1Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCKTRBS-OD
Plasmid#102924PurposepCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetOAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pO3
Plasmid#193748PurposeExpresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPAAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-TetO-QF_T7-LacO-TetR_p15A
Plasmid#171659PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.DepositorInsertT7-LacO-TetR-T7 Stop
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT002
Plasmid#182712PurposeaTc inducible dCasRx-IF1DepositorInsertdCasRx
UseCRISPRTags3x(GGGS)-Escherichia Coli Initiation Factor 1ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas2B
Plasmid#82373PurposedCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2E
Plasmid#82376PurposedCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2A
Plasmid#82372PurposedCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCas34
Plasmid#82386PurposesgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFW6000
Plasmid#224215PurposeBacteroides genomic editing tool; aTc inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry
Plasmid#221143PurposecdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)DepositorInsertcdGreen-Matry
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP NoStarts
Plasmid#101342PurposeAll ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACGDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationNo methonines in GagPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry-blind
Plasmid#221181PurposecdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)DepositorInsertcdGreen-Matry(blind)
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
UseTagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only