We narrowed to 2,461 results for: T2A

1. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Promoter

   EF1 alpha

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2-Scal-T2A-EGFP-Puro

   Plasmid

   #234949

   Purpose

   Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHDR-FOXL2-T2A-tdTomato-PuroTK

   Plasmid

   #192892

   Purpose

   HDR donor for inserting FOXL2-T2A-tdTomato reporter

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2-tdTomato (FOXL2 Human)
   

   Use

   CRISPR

   Mutation

   T2A-tdTomato fluorescent reporter

   Promoter

   PGK

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EFS-EGFP-T2A-Bsd

   Plasmid

   #193198

   Purpose

   Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-UBC-hCD47-T2A-GFP

   Plasmid

   #205458

   Purpose

   lentiviral plasmid for expression of human CD47

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD47 (CD47 Human)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CMV-mCherry-T2A-Puro

   Plasmid

   #72264

   Purpose

   Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

   Depositor

   Kazuhiro Oka

   Insert

   mCherry-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1-copGFP-T2A-Puro

   Plasmid

   #72263

   Purpose

   Express copGFP and puromycin resistance gene under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   copGFP-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. TRE-UBC-mCD47-T2A-GFP

   Plasmid

   #205449

   Purpose

   lentiviral plasmid for expression of mouse CD47

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD47 (Cd47 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

    Plasmid

    #244218

    Purpose

    Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

    Plasmid

    #244204

    Purpose

    Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

    Plasmid

    #244219

    Purpose

    Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-UBC-hCD55-T2A-GFP

    Plasmid

    #205459

    Purpose

    lentiviral plasmid for expression of human CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD55 (CD55 Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDNR-mCherry-T2A-3xPuroR-Frame 0

    Plasmid

    #206991

    Purpose

    Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

    Plasmid

    #168998

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Unspecified

    Tags

    EGFP and mRFP1

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-dCas9VP192-T2A-EGFP-shP53

    Plasmid

    #69535

    Purpose

    Episomal plasmid encoding dCas9VP192 and p53 shRNA

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-GFP-shp53
    

    Use

    CRISPR

    Tags

    EGFP

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. P-rTetO-EGFP-T2A-RPL22-3xHA

    Plasmid

    #170326

    Purpose

    Tetracycline-inducible expression of HA and EGFP tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SpCas9(D10A)-T2A-EGFP

    Plasmid

    #221230

    Purpose

    Express nCas9 nickase (D10) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-T2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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