We narrowed to 1,198 results for: e.coli
-
Plasmid#53125Purposeretroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertlacZ (lacZ E.coli)
UseRetroviral; Expression in chicken cellsPromoterSNV (spleen necrosis virus)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1070
Plasmid#53002PurposepCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsDsrA sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1757
Plasmid#53037PurposePconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialPromoterPconNoHindM2 and pConNoHindAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
Tags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available SinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUB11
Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
Tags3xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT22-GluCNZ-6His
Plasmid#213504PurposeDesigned for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.DepositorInsertGlucanase (glucan endo-1,3-beta-glucosidase)
Tags6His tagExpressionBacterialMutation1-36 Signal Sequence DeletionPromoterT7Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDC2
Plasmid#228779PurposeProtein expression plasmid for YTH domain of YTHDC2 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDC1
Plasmid#228778PurposeProtein expression plasmid for YTH domain of YTHDC1 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
Tags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
ExpressionBacterialPromoterpj23119Available SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-hRanGAP1
Plasmid#50941Purposeexpression of full length untagged human RanGAP1 in E.coliDepositorAvailable SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationW22HPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR
Plasmid#24584DepositorInsertpADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)
Plasmid#61929PurposeExpression of hUCH37 (ISF1) in E.coliDepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
ins:CFP-NTR
Plasmid#66593PurposeSpecific and conditional cell ablation of beta cells in the zebrafish pancreasDepositorInsertInsulin promotor driving CFP-nitroreductase
UseZebrafish expressionTagsCFP fused with N-terminus of nitroreductasePromoterZebrafish Insulin promoter (approximately 1.2kb)Available SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only