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pLO101

Plasmid

#90221

Purpose

Complements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_07102

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLO102

Plasmid

#90222

Purpose

Complements mutations in Aspergillus nidulans gene choA (AN2154) (mutants require choline)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_02347

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR eGFPCRE L1-R5

Plasmid

#43960

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

eGFPCre

Use

Multisite gateway entr clone

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pRoTnsABCDQ

Plasmid

#178772

Purpose

Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII

Plasmid

#80909

Purpose

Phagemid (encodes cI4A5C6G7G and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5C6G7G

Expression

Bacterial

Mutation

238G to 238D (please see depositor comment below)

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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mRFP1-1

Plasmid

#63816

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR IRES L5-L4

Plasmid

#43964

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

IRES

Use

Multisite gateway entr clone

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA1

Plasmid

#170088

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mRFP1-9

Plasmid

#63818

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2T

Plasmid

#62258

Purpose

expression of A2T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR pA L3-L2

Plasmid

#43966

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

pA

Use

Multisite gateway entr clone

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1T

Plasmid

#62256

Purpose

expression of A1T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4NT

Plasmid

#62261

Purpose

expression of A4NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3T

Plasmid

#62260

Purpose

expression of A3T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3NT

Plasmid

#62259

Purpose

expression of A3NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(F29K)

Plasmid

#245222

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

F29K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(L43F)

Plasmid

#245224

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

L43F

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletKm

Plasmid

#206820

Purpose

Template for hlpA-mScarlet-I amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenSpc

Plasmid

#206817

Purpose

Template for hlpA-mneongreen amplification associated with spectinomycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletEry

Plasmid

#206819

Purpose

Template for hlpA-mScarlet-I amplification associated with erythromycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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