We narrowed to 4,838 results for: phy

1. pcDNA3-S586A-VLCAD-FLAG

   Plasmid

   #55824

   Purpose

   Expresses S586A VLCAD mutant in mammalian cells

   Depositor

   Yoshimi Homma

   Article

   Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

   Insert

   Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   changed Serine 586 toAlanine

   Promoter

   CMV

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPKm-230

   Plasmid

   #90499

   Purpose

   pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tFD and mito-tFNR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-mkgDUX4mal*leu*

   Plasmid

   #21173

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Apr 9. ():.

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   First point mutation at nucleotide 5114 (numberin…

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pCS2-mkgDUX4mal*mqg*

   Plasmid

   #21174

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Apr 9. ():.

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   First point mutation at nucleotide 5114 (numberin…

   Promoter

   Available since

   Aug. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

5. BII-CMV-AfmW3A

   Plasmid

   #133402

   Purpose

   Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Afamin and Wnt3a (Afm, Wnt3a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2R3a-VenusYFP-3AT

   Plasmid

   #71269

   Purpose

   Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   VenusYFP
   

   Use

   Gateway

   Tags

   4xGly linker and T3A pea Pisum sativum ribulose-1…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-PHFF

   Plasmid

   #100280

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   

   Use

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BII-ChBtW-dnTCF4-ires-GFP

   Plasmid

   #133378

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   dnTCF4 (TCF4 Human)
   

   Use

   Tags

   2x Flag

   Expression

   Mammalian

   Mutation

   deltaN31

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-GFP-IMP2-2_antisense-control

   Plasmid

   #42174

   Depositor

   Alexandra Kiemer

   Article

   Kessler et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

    Plasmid

    #99261

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    Y705F (contains a silent mutation: nucleotide 271…

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-APLP1ICD-IRES-hrGFP

    Plasmid

    #107546

    Purpose

    AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    APLP1ICD (APLP1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177360

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsine promoter and U6 promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177365

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-syn-AICD-IRES-hrGFP

    Plasmid

    #107548

    Purpose

    high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD (APP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    human synapsin

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-AICD-NLS-IRES-hrGFP

    Plasmid

    #107543

    Purpose

    AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD-NLS (APP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-AICD-NES-IRES-hrGFP

    Plasmid

    #107544

    Purpose

    AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD-NES (APP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSC-NGN2-IRES-ISL1-T2A-LHX3

    Plasmid

    #221814

    Purpose

    Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

    Depositor

    Baojin Ding

    Article

    Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

    Inserts

    NEUROG2 (NEUROG2 Human)
    ISL1 (ISL1 Human)
    LHX3 (LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge