We narrowed to 9,235 results for: mel

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pRedCm

Plasmid

#176225

Purpose

LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaB and PL

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAdEasy-Msln-iCre-HA-Flag

Plasmid

#31305

Depositor

William Pu

Article

Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

Insert

Mesothelin (MSLN Human)

Use

Adenoviral

Tags

Flag and HA

Available Since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Lyn11-BirA*-HA

Plasmid

#214474

Purpose

Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

BirA(R118G)

Use

Lentiviral; Gateway

Tags

HA tag and Lyn11 (Plasma Membrane Localization Se…

Expression

Mammalian

Mutation

R118G

Promoter

EF-1a

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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ERroGFP_iE_C204Q_pCDNA3

Plasmid

#48629

Purpose

Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERroGFP

Tags

FLAG

Expression

Mammalian

Mutation

insertion E147B, C204Q

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-PpAPOBEC1 R33A

Plasmid

#138345

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1 R33A

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1 R33A

Use

Synthetic Biology

Tags

BP-NLS

Mutation

Change of Arg 33 to Ala.

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11

Plasmid

#172068

Purpose

Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

Plasmid

#124705

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_561-SV40-GFP

Plasmid

#75139

Purpose

Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_561

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEBFP2-NLS

Plasmid

#176682

Purpose

Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EBFP2

Expression

Insect

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(1,20,6)-ADAR2(E488Q)

Plasmid

#124621

Purpose

Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2(E488Q)

Use

AAV

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC3

Plasmid

#205062

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 3 aa

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

Plasmid

#124626

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD (E1008Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Hoxc6

Plasmid

#45553

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc6 (Hoxc6 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pUBC_V5_MS2-mVenus

Plasmid

#107606

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

MS2-mVenus

Use

Synthetic Biology

Tags

V5 and mVenus

Mutation

MS2: V75E/A81G

Promoter

human UBC

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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