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[pSK478] pLJC5 HA-Depdc5(P)

Plasmid

#109347

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant P

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

YDLLP(775-779)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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HMA-APOBEC1-Nb

Plasmid

#226012

Purpose

For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

Depositor

Eugene Yeo

Article

Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

Insert

APOBEC1 (Apobec1 Rat)

Tags

His-MBP and antiRabbit-nanobody

Expression

Bacterial

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR3c-V5/HIS

Plasmid

#201108

Purpose

expression of human FGFR3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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PIP2-GFP(ST) sensor

Plasmid

#170356

Purpose

GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Article

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

GFP(ST)-PH (PLCD1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pZY101

Plasmid

#73932

Purpose

This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Insert

bar

Expression

Plant

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-ARF

Plasmid

#89123

Purpose

mammalian expression of p14-hARF with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PHC3

Plasmid

#45262

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PHC3 (PHC3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
FUW-M2-PAK2 wt

Plasmid

#31680

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-221-PGK-GFP-Blast

Plasmid

#31519

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MSCV miRNA control plasmid

Plasmid

#31508

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Type

Empty backbone

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRK5_mCherry_P2AT2A_GFP-nb

Plasmid

#238232

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Spike

Plasmid

#168951

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Spike (S )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OT1.0

Plasmid

#185384

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-His6-1_HBD

Plasmid

#171869

Purpose

Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-CAG::Mitbow

Plasmid

#158994

Purpose

Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Mitbow

Tags

The default expressed EBFP2 is fused to H2B; oth…

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMM643

Plasmid

#112533

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Mutation

A17P

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS3

Plasmid

#120822

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS3 ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS5

Plasmid

#120825

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS5 ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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