We narrowed to 58,554 results for: tra

1. pTAG4

   Plasmid

   #176867

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_4
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTSB-FICAT_Payload-Esp3I

   Plasmid

   #179380

   Purpose

   Transposon vector prepared for Esp3I golden gate cloning of Transgene

   Depositor

   Marc Güell

   Article

   Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CSM160

   Plasmid

   #105684

   Purpose

   Plasmid containing an RFP dropout used to construct randomized PAM library

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   RFP Golden Gate dropout
   

   Expression

   Bacterial

   Promoter

   Bba_R0040 TetR Promoter

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEDJ-87

   Plasmid

   #163085

   Purpose

   MarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5

   Depositor

   Michael Jensen

   Article

   Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

   Insert

   VPR
   

   Tags

   MCP

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDM_N1_400

   Plasmid

   #170474

   Purpose

   To amplify transcription template containing LacI operators

   Depositor

   Laura Finzi

   Article

   Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

   Insert

   T7A1, O-, O1, Lambda t1 in modified lacZ fragment
   

   Expression

   Bacterial

   Mutation

   diminished hairpins near O1 and diminished latent…

   Promoter

   T7A1

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAra-tns

   Plasmid

   #170632

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTnsABC
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27Linear-2, PolyU(0)

   Plasmid

   #166994

   Purpose

   Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0)
   

   Expression

   Mammalian

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Linear-1, PolyU(0)

   Plasmid

   #166992

   Purpose

   Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0)
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GLY, Linear-3, PolyU(1)

   Plasmid

   #166988

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1)
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GP821

    Plasmid

    #164552

    Purpose

    Expresses Smo-FLAG K434R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K434R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    One of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEL2298

    Plasmid

    #164603

    Purpose

    Expression and Complementation plasmid for MlaD (A17F, A20F, V24F)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

    Insert

    MlaFEDCB
    

    Expression

    Bacterial

    Mutation

    MlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GP823

    Plasmid

    #164554

    Purpose

    Expresses Smo-FLAG K448R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K448R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    One of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GP822

    Plasmid

    #164553

    Purpose

    Expresses Smo-FLAG K444R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K444R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    One of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GP806

    Plasmid

    #164534

    Purpose

    Expresses Smo noK-FLAG-Ub

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened noK (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-Ub

    Mutation

    All 21 cytoplasmic Lysines of Smoothened changed …

    Promoter

    CMV

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBzCas13b-gRNA-2

    Plasmid

    #164859

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-2
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBzCas13b-gRNA-3

    Plasmid

    #164860

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-3
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLsCas13a-gRNA-3

    Plasmid

    #164867

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-3
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLsCas13a-gRNA-2

    Plasmid

    #164866

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-2
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCL33-decoder

    Plasmid

    #159451

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Pfus1mut

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGST-pol1-N1

    Plasmid

    #160988

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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