We narrowed to 24,960 results for: LARS

1. ER-moxMaple3

   Plasmid

   #120885

   Purpose

   mammalian expression of ER localized moxMaple3

   Depositor

   Erik Snapp

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Insert

   moxMaple3
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTmpKm

   Plasmid

   #176227

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNTI819

   Plasmid

   #216628

   Purpose

   Cal-ID (V5) for mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCL28-decoder

   Plasmid

   #159450

   Purpose

   Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.

   Depositor

   Joerg Stelling

   Article

   Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

   Insert

   PlexAtet-LexA-ER-B112-tCyc1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PlexAtet

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCL66-decoder

   Plasmid

   #159453

   Purpose

   Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

   Depositor

   Joerg Stelling

   Article

   Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

   Insert

   Pfus1mut-tetR-nls-malE-tCyc1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pfus1mut

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD143

   Plasmid

   #119880

   Purpose

   Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_dCas9sth1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV_POWV_PrME

   Plasmid

   #207299

   Purpose

   Mammalian expression of Tick-borne powassan virus PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   POWV_PrME (POLY Tick-borne powassan virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Level 1 P4 TaU6 guide acceptor

   Plasmid

   #165600

   Purpose

   GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Level 1 P3 TaU6 guide acceptor

   Plasmid

   #165599

   Purpose

   GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCMV_MARV_GP

    Plasmid

    #207292

    Purpose

    Mammalian expression of Lake victoria Marburgvirus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    MARV_GP (GP Lake victoria Marburgvirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEM371 - ce-unc-119 array integration

    Plasmid

    #182640

    Purpose

    Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNTI820

    Plasmid

    #216629

    Purpose

    cpTurboID (V5) for mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT-Pan

    Plasmid

    #99638

    Purpose

    EGFP-mCherry control reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Use

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    chicken actin

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pHH0103_PLK1_Pkinase

    Plasmid

    #109933

    Purpose

    Protein expression and purification of PLK1_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PLK1_Pkinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-FLAG-SENP3

    Plasmid

    #115521

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

    Insert

    SENP3 (Senp3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEYFP-p23

    Plasmid

    #108225

    Purpose

    Mammalian expression vector for EYFP fusion to human p23

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EYFP-p23 (PTGES3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCX3-ACTG2_R257C

    Plasmid

    #228911

    Purpose

    Expresses mutant R257C smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    R257C

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJCX3-ACTG2_WT

    Plasmid

    #228910

    Purpose

    Expresses wildtype smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lv EF1a MS2-Fkbp2x 2A Hygro

    Plasmid

    #102807

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-Fkbp2x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. LT1_33_pTEFmut8-mCherry

    Plasmid

    #177706

    Purpose

    Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEFmut8-mCherry
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-SENP5

    Plasmid

    #34555

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    SENP5 (SENP5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

23. Friend MLV Env YFP

    Plasmid

    #1820

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    Envelope (env MuLV)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDae029 (eMutaT7[TadA-8e])

    Plasmid

    #187620

    Purpose

    Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    TadA-8e-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTmpSm

    Plasmid

    #176228

    Purpose

    A template plasmid for amplification of the cI-hok-strA cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, strA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. betaRBPIG

    Plasmid

    #16422

    Depositor

    Juergen Hescheler

    Article

    Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

    Insert

    loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pGFP Cas

    Plasmid

    #50729

    Purpose

    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cas (Bcar1 Rat)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHCMV_CHIKV_E1E2

    Plasmid

    #207261

    Purpose

    Mammalian expression of Chikungunya virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHCMV_YFV_PrME

    Plasmid

    #207315

    Purpose

    Mammalian expression of Yellow fever virus PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    YFV_PrME (POLY Yellow fever virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge