We narrowed to 11,999 results for: SHA

Showing: 8161 - 8190 of 11999 results

Results per page:

Sort results by:

shTCF7L2 # 2

Plasmid

#42558

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-1574/+120

Plasmid

#35539

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG426GAL-KUNV_NS2B-GS-NS3

Plasmid

#203506

Purpose

Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

KUNV NS2B-GS-NS3

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-moxGFP-Puro-H3C2

Plasmid

#207783

Purpose

Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DCP2

Plasmid

#155499

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DCP2 (DCP2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500

Plasmid

#92317

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-his-Gluc-iLID

Plasmid

#172096

Purpose

Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - iLID

Expression

Bacterial

Mutation

Gluc Mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

Plasmid

#58275

Purpose

mammalian expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
mKate1.3-Fibrillarin-7

Plasmid

#56084

Purpose

Localization: Nucleoli, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

Fibrillarin (FBL Human)

Tags

mKate1.3 (aka mKate2)

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pXPR_BRD111

Plasmid

#78166

Purpose

Expresses Cas9 with the pLX311 backbone

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Cas9

Use

Lentiviral

Tags

3xFLAG and NLS

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
Clover-Mito-7

Plasmid

#56529

Purpose

Localization: Mitochondria, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Mito (COX8A Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFUW-tetO-GFI1

Plasmid

#139813

Purpose

doxycycline-inducible expression of Human GFI1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

GFI1 (GFI1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P2L- Please see depositor comment below

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNeuLite PEA3 mt

Plasmid

#16248

Depositor

Mien-Chie Hung

Article

Xing et al Nat Med. 2000 Feb . 6(2):189-95.

Insert

Her-2/neu promoter (ERBB2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

PEA3 mt: PEA3 motif AGGAAG changed to AGCTCG

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
qTAG-N-Blast-sTagRFP-TUBA1B

Plasmid

#207768

Purpose

Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLEX304-ARF5-mNeonGreen

Plasmid

#162025

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hPMCA2xb

Plasmid

#47751

Purpose

mammalian expression of mCherry tagged hPMCA2, xb splice variant

Depositor

Emanuel Strehler

Article

Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

Insert

PMCA2x/b (ATP2B2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

xb splice variant

Promoter

CMV

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only
pLV V5-TbID-emerin

Plasmid

#175099

Purpose

Lentiviral expression of V5-TbID-tagged mouse Emd

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5-TbID

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

Plasmid

#135565

Purpose

An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 rat SST
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

SYN1 and hSYN1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS04 [myo-3p::bPGC::SL2::mCherry]

Plasmid

#168172

Purpose

Expression of bPGC in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

bPGC

Expression

Worm

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SMN1-1

Plasmid

#156022

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SMN1 (SMN1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM2_mCherry_Mesp2

Plasmid

#72693

Purpose

Doxycyline regulated expression of Mesp2 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 2 (Mesp2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PARD6A_PDZ_1

Plasmid

#103946

Purpose

Protein expression and purification of TAX INTERACTION PROTEIN PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PARD6A (PARD6A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Gluc-EL222-his

Plasmid

#172097

Purpose

Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - EL222

Expression

Bacterial

Mutation

Gluc mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
integrin beta6 777T pcDNA1 neo

Plasmid

#13582

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Expression

Mammalian

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge