We narrowed to 18,064 results for: Gateway

1. AIRE_pCSdest

   Plasmid

   #53891

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   AIRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pLentiX2-PURO-shGFP

   Plasmid

   #61230

   Purpose

   Lentivirus expressing shRNA to GFP

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pYZ172

   Plasmid

   #98409

   Purpose

   Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Inserts

   upstream homologous region to delete lys9 in genome by recombination
   downstream homologous region to delete lys9 in genome by recombination
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-cT3G-cERP2-MEIOC

   Plasmid

   #222562

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   MEIOC (C17orf104 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR4-GST 6P-1 (w487-1)

   Plasmid

   #17741

   Purpose

   Gateway entry vector for N-term GST fusion, frame 6P-1

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   GST

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA(MS2)_zeo insert c-MYC

   Plasmid

   #164636

   Purpose

   sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

   Depositor

   Günter Schneider

   Insert

   target sequence for c-Myc activation (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ1655

   Plasmid

   #51481

   Purpose

   miniMos transposable element with HygroR, R4-R3 Gateway cloning

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pE1B-C-F/Sox10-MCS4

   Plasmid

   #20243

   Depositor

   William Pavan

   Article

   Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

   Insert

   Sox10-MCS4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWS926

   Plasmid

   #123052

   Purpose

   LexA bacterial repressor

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   LexA-NLS [3a]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZmUBQ:Mla1

    Plasmid

    #132355

    Purpose

    Expression of the barley Mla1 NLR from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    HvMla1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid

    #189554

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid

    #189560

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-654

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCM167-Venus-VF-hADAM10(aa1-459)

    Plasmid

    #189559

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-459

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFF3_pCSdest

    Plasmid

    #53888

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    TFF3 (TFF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pE1B-C-F/Sox10-MCS7

    Plasmid

    #20246

    Depositor

    William Pavan

    Article

    Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

    Insert

    Sox10-MCS7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-cT3G-cERP2-DMC1

    Plasmid

    #222541

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    DMC1 (DMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

    Plasmid

    #163062

    Purpose

    Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    QuasAr2
    

    Use

    Gateway entry vector

    Tags

    mOrange2

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-EF1a-MegaGate-DD-Hygro

    Plasmid

    #175267

    Purpose

    MegaGate destination with C-terminal DD tag and hygromycin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Tags

    Destabilization Domain

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQCXIB CMV/TO DEST (w320-1)

    Plasmid

    #17400

    Purpose

    Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Retroviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC_N_SOX5

    Plasmid

    #187742

    Purpose

    MAC-tagged gene expression of human SOX5

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX5 (SOX5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQ151

    Plasmid

    #69302

    Purpose

    Gateway entry vector with pcoCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9D10A (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR/pSM2(CMV/TO) (w55-1)

    Plasmid

    #17388

    Purpose

    Gateway entry vector for miRNA expression under CMV/TO promoter

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    RNAi; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST-EGFP-TARG1

    Plasmid

    #172586

    Purpose

    For transient expression of N-terminal GFP-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFRT_TO_FlagHA_PRKRA

    Plasmid

    #106111

    Purpose

    Inducible gene expression of FlagHA-PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MAC_N_PAX7

    Plasmid

    #187750

    Purpose

    MAC-tagged gene expression of human PAX7

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    PAX7 (PAX7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCellFree_G03 WWP1

    Plasmid

    #67125

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHRW-CRY2olig-VHH(GFP)

    Plasmid

    #126023

    Purpose

    express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHRolig-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    CRY2 E490G

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR4-V5-LUC (w468-1)

    Plasmid

    #19135

    Purpose

    Gateway entry vector, V5-Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Entry vector

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ140

    Plasmid

    #99895

    Purpose

    Empty vector with attL5-attL2 recombination sites for Gateway Cloning

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR2B-dCas9-KRAB

    Plasmid

    #115547

    Purpose

    Entry cassette for dCas9-KRAB

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Insert

    dCas9-KRAB
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDEST51-LRRK2-R1441G

    Plasmid

    #25082

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Arginine 1441 mutated to Glycine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pWS481

    Plasmid

    #123029

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (6x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG425 GAL Pong TPase L418A, L420A

    Plasmid

    #145795

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock, Susan Wessler

    Article

    Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

    Insert

    Pong TPase L418A, L420A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L418A, L420A

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGWT35S-Cerulean-SKL

    Plasmid

    #194048

    Purpose

    Transient expression of Cerulean-SKL in plant cell (Peroxisome)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    Cerulean
    

    Use

    Tags

    Ser-Lsy-Leu

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pCellFree_G03 TFAP2B

    Plasmid

    #67092

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pQLinkHD_EBFP2-LSSmOrange

    Plasmid

    #118856

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2-LSSmOrange
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-mOvol1

    Plasmid

    #120359

    Purpose

    Gateway entry vector containing mouse Ovol1.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge