163,606 results

1. pBSV2_PflaB-mCherryBb

   Plasmid

   #118243

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tau (P301L)-VC

   Plasmid

   #87633

   Purpose

   expresses hTau fused to the C-terminal part of Venus

   Depositor

   Tiago Outeiro

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Mutation P301L

   Promoter

   CMV

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY1513 AAVS1 sgRNA

   Plasmid

   #234832

   Purpose

   sgRNA for AAVS1 STITCHR insertion

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   AAVS1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHA-VRC01-scFv

   Plasmid

   #44874

   Depositor

   Dane Wittrup

   Article

   Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

   Insert

   VRC01 scFv
   

   Use

   Tags

   HA and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-TBP

   Plasmid

   #26674

   Depositor

   Sui Huang

   Article

   Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

   Insert

   TBP (TBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. MIB2-FLAG

   Plasmid

   #37117

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-roxSTOProx-ZsGreen

   Plasmid

   #51274

   Purpose

   Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   roxSTOProx-ZsGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCH67 (crRNA expression vector)

   Plasmid

   #217337

   Purpose

   CROP-seq crRNA expression vector with GFP and puro marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234452

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB517

    Plasmid

    #74859

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPaint-NanoLuc

    Plasmid

    #67178

    Purpose

    CRISPaint universal donor for NanoLuc fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hACE2

    Plasmid

    #145033

    Purpose

    Express human ACE2 with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    human ACE2 (ACE2 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEGFP-N1-GCaMP6m-Xn

    Plasmid

    #118976

    Purpose

    Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIpA102-G-HLA-A2_GFP

    Plasmid

    #85162

    Purpose

    for mRNA expression of HLA.A2-EGFP

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Mrna expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNDGG054

    Plasmid

    #231323

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. Prig-3::CD4::spGFP1-10

    Plasmid

    #65829

    Purpose

    For membrane contact

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
    

    Use

    Tags

    spGFP1-10

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCPP5372

    Plasmid

    #128733

    Purpose

    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Prig-3::CD4::spGFP11

    Plasmid

    #65830

    Purpose

    For membrane contact

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
    

    Use

    Tags

    spGFP11

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. dCas9-EGFP

    Plasmid

    #232761

    Purpose

    Expresses dCas9-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-RT003-GPM

    Plasmid

    #163383

    Purpose

    To express sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    GFP-PDGFRtm-2A-mCherry (GPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmCherry-Akt1(WT)

    Plasmid

    #86631

    Purpose

    Expresses mCherry-tagged human Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge