176,531 results

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MXS_BidirectionalCAG

Plasmid

#78175

Purpose

Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

Bidirectional CAG Promoter

Use

Synthetic Biology

Expression

Mammalian

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLH-sgRNA1-2XboxB

Plasmid

#75391

Purpose

sgRNA1-2XboxB

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XboxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-centrin-1

Plasmid

#72641

Purpose

The plasmid encodes centrin-1 with an N-terminal EGFP tag

Depositor

Michel Bornens

Insert

centrin-1 (CETN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ133A

Plasmid

#69275

Purpose

Golden Gate entry vector; 3rd gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCITF-KCC2

Plasmid

#61404

Purpose

Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

Depositor

Franck Polleux

Publication

Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

Insert

KCC2 (SLC12A5 Human)

Tags

IRES-tdTomato-Flag

Expression

Mammalian

Promoter

CAG

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Publication

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pYLsgRNA-OsU6b

Plasmid

#66196

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6b

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-BRDT

Plasmid

#65381

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRDT (BRDT Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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BiP-mCherry

Plasmid

#62233

Purpose

Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Publication

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mCherry-KDEL

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPHT-PKA

Plasmid

#60936

Purpose

Fluorescent FPX sensor for cAMP

Depositor

Anne Marie Quinn

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PKA FPX Sensor

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCherry-MAPTau-C-10

Plasmid

#55077

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

MAPTau (MAPT Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CoChR-GFP

Plasmid

#59070

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mApple-Alpha-V-Integrin-25

Plasmid

#54866

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Publication

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

mCherry

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mEmerald-Lysosomes-20

Plasmid

#54149

Purpose

Localization: Lysosome Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Lysosomes

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pMDC123SB-AtMIR390a-B/c

Plasmid

#51775

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Publication

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pVITRO1-M80-F2-IgG1/κ

Plasmid

#50383

Purpose

Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

human Gamma 1 heavy chain expression cassette with unknown specificity
human Kappa light chain expression cassette with unknown specificity

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available since

Jan. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK GFP Paxillin

Plasmid

#50529

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Insert

Paxillin (PXN Human)

Tags

GFP21

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pKIKOlacZCm

Plasmid

#46764

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Publication

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1+/ER-2mutAEQ

Plasmid

#45541

Depositor

Javier Alvarez-Martin

Publication

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

ER-2mutAEQ

Tags

ER targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Publication

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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BRD4

Plasmid

#38942

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD4 (BRD4 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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drebrin-YFP

Plasmid

#40359

Depositor

Phillip Gordon-Weeks

Publication

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Chick expression

Tags

eYFP

Promoter

chick B-actin promoter with CMV enhancer

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Publication

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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USP2

Plasmid

#36894

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP2 (USP2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Available since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles