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167,150 results

Showing: 8221 - 8250 of 167150 results

  1. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

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  3. p667-UBC-CDK12-V5-TurboID_IDG-K

    Plasmid
    #135244
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-TurboID (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135244-plasmid-map-sequence-id-311575

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  5. NSUN5

    Plasmid
    #25491
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NSUN5 (NSUN5 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pADH147

    Plasmid
    #90991
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90991-plasmid-map-sequence-id-180524

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  12. pJAT7

    Plasmid
    #204292
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204292-plasmid-map-sequence-id-405844

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  24. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209189-plasmid-map-sequence-id-419145

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  25. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183956-plasmid-map-sequence-id-363081

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  28. mito-meGFP

    Plasmid
    #172481
    Purpose
    meGFP targeted to the mitochondrial matrix with a human COX8 presequence
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Tags
    meGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    172481-plasmid-map-sequence-id-345344

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Showing: 8221 - 8250 of 167150 results