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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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SUMO-Twinkle 43-372

Plasmid

#205054

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Expression

Bacterial

Mutation

Lacks Mitochondrial localization signal and C ter…

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3

Plasmid

#203601

Purpose

Reporter plasmid carrying B4GALT3 3'UTR

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

nt 1800-2330 3'UTR

Promoter

SV40

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1

Plasmid

#203603

Purpose

Reporter plasmid carrying MAIP1 3'UTR

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23-Gja1prom

Plasmid

#188114

Purpose

Expresses luciferase under control of Gja1 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

Insert

Gja1 promoter (Gja1 Mouse)

Use

Luciferase

Promoter

Gja1 endogenous promoter up to gene start codon

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTdelta5cys

Plasmid

#207465

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTdelta5cys (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, L177D

Plasmid

#180649

Purpose

Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, L177D mutation, has siRNA resistan…

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT021-LAT_HASA-HaloTag-3x FLAG

Plasmid

#202505

Purpose

Express high ASA Lat mutant

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

LAT (LAT Human)

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

Several leucine subsitutions in transmembrane dom…

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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DNMT2Δ191-237

Plasmid

#198382

Purpose

Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

Depositor

Tanja Schirmeister

Article

Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

Insert

DNMT2 (TRDMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

deleted amino acids 191-237

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCYFIP1_T

Plasmid

#147578

Purpose

Insect Expression of DmCYFIP1

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1 (Sra-1 Fly)

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCYFIP1_T

Plasmid

#147585

Purpose

Insect Expression of DmCYFIP1

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1 (Sra-1 Fly)

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCYFIP1-4xmut_T

Plasmid

#147586

Purpose

Insect Expression of DmCYFIP1-4xmut

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1-4xmut (Sra-1 Fly)

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCYFIP1-K743E_T

Plasmid

#147587

Purpose

Insect Expression of DmCYFIP1-K743E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1-K743E (Sra-1 Fly)

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T

Plasmid

#147588

Purpose

Insect Expression of DmeIF4Ga_578-650-CG32016-E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4Ga_578-650-CG32016-E (eIF4G1 Fly)

Expression

Insect

Mutation

CG32016-E 5 silent and three no silent mutations …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-γ1

Plasmid

#197078

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-1 γ1 (Ap1g1 Mouse)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEE005

Plasmid

#176762

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

101

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEE015

Plasmid

#176772

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

207

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE017

Plasmid

#176774

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

209

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE022

Plasmid

#176779

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

214

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE024

Plasmid

#176781

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

301

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE025

Plasmid

#176782

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

302

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE026

Plasmid

#176783

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

303

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE010

Plasmid

#176767

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

202

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna3.1_mouseST6galI

Plasmid

#178435

Purpose

murine St6gal1 expression plasmid

Depositor

Deborah Fass

Article

Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

Insert

St6gal1 (St6gal1 Mouse)

Tags

6xHIS-TEV Cleavage Site

Expression

Mammalian

Mutation

Coding sequnce for amino acids 85-403

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo-W35A_TAF3_Phd

Plasmid

#179401

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo(W35A) - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W35A

Promoter

CAGGS

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A

Plasmid

#175140

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C211A

Plasmid

#175139

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C211A

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Hsd11b1-V5

Plasmid

#175147

Purpose

Lentiviral expression of mouse Hsd11b1-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Hsd11b1 (Hsd11b1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A, C211A

Plasmid

#175141

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A, C211A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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