We narrowed to 30,618 results for: ica

1. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Fon/ConN2cG

   Plasmid

   #234709

   Purpose

   pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

   Depositor

   David Ng

   Article

   Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

   Insert

   Fon Con N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK1_022

   Plasmid

   #123684

   Purpose

   Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-A4 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MTK1_024

   Plasmid

   #123686

   Purpose

   Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L2-C3 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMAL-C2X dAmp/Kana+

   Plasmid

   #154352

   Purpose

   Empty backbone vector

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MTK1_023

   Plasmid

   #123685

   Purpose

   Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L1- B1 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046959

   Plasmid

   #145722

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

    Plasmid

    #32474

    Purpose

    Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAMQ79G,Q139G-5HT3 HC
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #125692

    Purpose

    Global labeling of inhibitory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-myc3-NEDD8

    Plasmid

    #19943

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

13. MTK234_008

    Plasmid

    #123902

    Purpose

    Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    GFP dropout for multiTU destination vectors
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEN396-pCAGGS-TIR1-V5-2A-HygroR

    Plasmid

    #195102

    Purpose

    Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    TIR1
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJH1371

    Plasmid

    #199631

    Purpose

    Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GPI
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Avr2-GFP pZK538

    Plasmid

    #118011

    Purpose

    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

    Depositor

    FLW Takken

    Article

    et al Bio-protocol 8(24): e3114.

    Inserts

    dspAvr2
    mCherry
    

    Use

    Tags

    EGFP and HDEL

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMU-ERr

    Plasmid

    #61170

    Purpose

    Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtWAK2-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET12a-PAPI

    Plasmid

    #19961

    Depositor

    Jonathan Tait

    Article

    Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

    Insert

    Annexin V (ANXA5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO/cmycDrosha

    Plasmid

    #10828

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

    Insert

    Drosha (DROSHA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p2H12Matry

    Plasmid

    #221143

    Purpose

    cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen-Matry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Clover-Geminin(1-110)

    Plasmid

    #83915

    Purpose

    Fluorescent probe for M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-hfYFP

    Plasmid

    #186523

    Purpose

    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28b-GBP-MTn

    Plasmid

    #154363

    Purpose

    Bacterial expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBbA5c-MevT-MBIS

    Plasmid

    #35150

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5,

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GI-Gal4 DBD

    Plasmid

    #42500

    Purpose

    GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

    Depositor

    Ricardo Dolmetsch

    Article

    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

    Insert

    GIGANTEA fused with Gal4 DBD
    

    Use

    Tags

    Gal4 DNA binding domain (DBD)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pK-myc-Par3b

    Plasmid

    #19388

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Insert

    Partitioning-defective protein 3b (PARD3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Full length, isoform b.

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSB700 lenti gRNA Blasto

    Plasmid

    #167904

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSb_init containing the MBP sybody Sb_MBP#1

    Plasmid

    #132699

    Purpose

    expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    sybody_MBP#1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP-hsRPA14 (MSW#503)

    Plasmid

    #208069

    Purpose

    Expression of GFP-tagged hsRPA3 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA3 (RPA3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p3E-FRT-KanR-FRT pA

    Plasmid

    #82601

    Purpose

    3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRT-Kan-FRT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pADV-Sec61b-TurboID

    Plasmid

    #179143

    Purpose

    Recombinant adenoviral plasmid for Sec61b-TurboID expression

    Depositor

    Jae Myoung Suh

    Article

    Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

    Insert

    Sec61b-V5-TurboID
    

    Use

    Adenoviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EGFP-Rab13 in pCSDEST2

    Plasmid

    #140879

    Purpose

    Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab13 (rab13 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBabepuroBAF180

    Plasmid

    #41078

    Depositor

    Ramon Parsons

    Article

    Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

    Insert

    BAF180 (PBRM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations: 3595 a to g; 4981 a to t; 587…

    Promoter

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC-94N

    Plasmid

    #53281

    Purpose

    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXJ-His-PDCD1LG2

    Plasmid

    #232660

    Purpose

    transient expresses PD-L2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    PDCD1LG2 (PDCD1LG2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FLAG-SENP6m

    Plasmid

    #18716

    Depositor

    Edward Yeh

    Article

    Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

    Insert

    FLAG-SENP6m (SENP6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C1030A

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only