We narrowed to 7,530 results for: Ski;

1. pX330-Elf5 gRNA

   Plasmid

   #128836

   Purpose

   gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   mElf5 gRNA (Elf5 Mouse)
   

   Use

   CRISPR

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX311 HRAS

   Plasmid

   #117749

   Purpose

   Open reading frame vector encoding HRAS

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   RASH1 (HRAS Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Closed vector so will not read through the V5 reg…

   Promoter

   EF1a

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-CRT-Fc FL

   Plasmid

   #221853

   Purpose

   Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   calreticulin (CALR Human)
   

   Tags

   Human IgG1Fc tag

   Expression

   Mammalian

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZLblasti-HA-ERK2 R67S

   Plasmid

   #53172

   Purpose

   Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human, Mustard Weed)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R67S

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pExp-RBD-CHis

   Plasmid

   #195002

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Tags

   His8

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIN40C.SFFV.ARID3a.IRES.GFP

   Plasmid

   #169300

   Purpose

   Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   ARID3A (ARID3A Human)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   SFFV

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #174666

   Purpose

   Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Arid3a-3xFLAG (Arid3a Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139088

   Purpose

   Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   xCas9 3.7
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   A262T, R324L,S409I, E480K, E543D, M694I, E1219V

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTEE

   Plasmid

   #159748

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetO-Gata3

    Plasmid

    #128824

    Purpose

    dox-dependent expression of mouse Gata3

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-TetO-Eomes

    Plasmid

    #128825

    Purpose

    dox-dependent expression of mouse Eomes

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral

    Mutation

    Shorter isoform

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Clone 16 - 3 HK02 pFUSE-IgG-human kappa VL-CH1 hole-clone2

    Plasmid

    #192185

    Purpose

    Clone 16 (HK02) pFUSE-IgG-human kappa VL-CH1 hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 light chain (HK02)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZLblasti-HA-ERK2 D321N

    Plasmid

    #53173

    Purpose

    Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human, Mustard Weed)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D321N

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. lentiCRISPR v2-sgBAK-2

    Plasmid

    #210132

    Purpose

    knock out BAK in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Bcl-2 homologous antagonist/killer (BAK1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgBAK-1

    Plasmid

    #210131

    Purpose

    knock out BAK in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Bcl-2 homologous antagonist/killer (BAK1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5'-3xFLAG Chtop-exon minimal CMV pCDNA5

    Plasmid

    #212080

    Purpose

    LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop-exon (Chtop Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-PNKP

    Plasmid

    #215137

    Purpose

    Expresses mCherry-PNKP in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PNKP (PNKP Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139091

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CCND2 targeting gRNA

    Plasmid

    #215318

    Purpose

    Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CCND1 targeting gRNA

    Plasmid

    #215153

    Purpose

    Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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