We narrowed to 60,645 results for: promoter

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T5-sgRNA

Plasmid

#113655

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter T5 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

T5

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 Bt δCOP

Plasmid

#73871

Purpose

Bos taurus Arcn1

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 Promoter- COPD

Expression

Yeast

Promoter

Ret2 Promoter

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_mP_CMV

Plasmid

#99313

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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elephant.dPRL

Plasmid

#35255

Depositor

Gunter Wagner

Article

Emera et al Mol Biol Evol. 2011 Sep 4.

Insert

dPRL promoter for elephant

Use

Luciferase

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
M13 pCSKA-CAT

Plasmid

#17150

Depositor

Randall Moon

Insert

Cytoskeletal actin promoter

Use

Xenopus expression

Tags

CAT

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:NLuc

Plasmid

#118065

Purpose

Transcriptional unit for constitutive expression of NLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:NLuc:bGH

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GFP

Plasmid

#39289

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL2B -78/+64

Plasmid

#24947

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
GL4.10 E2E1- Prg4 promoter-luciferase

Plasmid

#195035

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.10 E3E2E1-Prg4 promoter-luciferase

Plasmid

#195036

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

Plasmid

#195037

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_FB

Plasmid

#78668

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

Plasmid

#132426

Purpose

Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

Ubiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDL11c

Plasmid

#17833

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Mutation

The argB_1626 mutation consists of two sequential…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pBP-P_rhaS-PRhaB

Plasmid

#204076

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

rhaS activator and PRhaB rhamnose-inducible promoter

Expression

Bacterial

Mutation

None

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_GB

Plasmid

#78669

Purpose

36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_EB

Plasmid

#78667

Purpose

36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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