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pPN170

Plasmid

#91599

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN171

Plasmid

#91600

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN190

Plasmid

#91577

Purpose

Express sgRNA targeting human BRINP2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BRINP2 (FAM5B Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.2.mKO2

Plasmid

#85210

Purpose

TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
FNE2-A72

Plasmid

#44608

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2-A72 (Ets2 Mouse)

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

THR-72 converted to Alanine

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pLENTICRISPR-ACIN1-sgRNA1

Plasmid

#165925

Purpose

CRISPR-mediated gene perturbation (knock-out) of ACIN1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

ACIN1 (ACIN1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-ACIN1-sgRNA1

Plasmid

#165926

Purpose

CRISPR-mediated gene perturbation (knock-out) of ACIN1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

ACIN1 (ACIN1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-HK2-sgRNA1

Plasmid

#165929

Purpose

CRISPR-mediated gene perturbation (knock-out) of HK2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

HK2 (HK2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-HK2-sgRNA2

Plasmid

#165930

Purpose

CRISPR-mediated gene perturbation (knock-out) of HK2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

HK2 (HK2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-LUC7L2-sgRNA1

Plasmid

#165935

Purpose

CRISPR-mediated gene perturbation (knock-out) of LUC7L2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-LUC7L2-sgRNA2

Plasmid

#165936

Purpose

CRISPR-mediated gene perturbation (knock-out) of LUC7L2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-puro-LUC7L2-3xFLAG

Plasmid

#165952

Purpose

Overexpression of LUC7L2.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Tags

3x-FLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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pWPI-Neo-LUC7L-3xFLAG

Plasmid

#165955

Purpose

Overexpression of LUC7L.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.Tau2N4R

Plasmid

#226396

Purpose

Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pU6_empty-sgRNA(PP7)

Plasmid

#232432

Purpose

empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged sgRNA scaffold driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+MLS-HyPer7

Plasmid

#136470

Purpose

Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Tandem mitochondrial targeting signal of cytochro…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCUU

Plasmid

#159747

Purpose

Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

Depositor

Wolfgang Lukowitz

Article

Angulo et al bioRxiv

Insert

gRNA scaffold

Use

CRISPR

Expression

Plant

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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