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W118-1_hTGM2-V5

Plasmid

#180413

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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CPSF5

Plasmid

#155480

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF5 (NUDT21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-2A-3xFLAG-SBP-2A

Plasmid

#159584

Purpose

Luciferase reporter for ribosome pausing control

Depositor

Shintaro Iwasaki

Article

Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

Inserts

2A-3xFLAG-SBP-2A
EIF2S3 5UTR

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Expression

Bacterial and Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-MAHS

Plasmid

#90034

Purpose

To express GFP-tagged MAHS in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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mWasabi-ER-3

Plasmid

#56501

Purpose

Localization: Endoplasmic Reticulum, Excitation: 493, Emission: 509

Depositor

Michael Davidson

Insert

ER (CALR Human)

Tags

mWasabi

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFS177

Plasmid

#12380

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

human equilibrating nucleoside transporter 1 (SLC29A1 Human)

Expression

Yeast

Mutation

N81S and M84V

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pBT250_(pCA-G-intron(Neo)-tTA2)

Plasmid

#36876

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB301_ZFcharm_Kv1_Prnp_SPM

Plasmid

#220840

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

Plasmid

#200277

Purpose

Characterisation of the sialic acid-responsive circuit with fluorescent reporter

Depositor

Matthew Chang

Article

Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

Inserts

CadC
NanR

Expression

Bacterial

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPSup_35Senh(fwd)_35Spr+synJ

Plasmid

#149422

Purpose

Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Insert

35S core enhancer

Expression

Plant

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

Plasmid

#91917

Purpose

Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LweCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CG_WT

Plasmid

#81843

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway Cloning

Mutation

E2G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PDZD7-TwinStrepII

Plasmid

#83198

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Article

Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

Insert

PDZD7 (Pdzd7 Mouse)

Tags

Twin Strep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN

Plasmid

#73365

Purpose

Expresses myc tagged XPLN in mammalian cells

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
bio-CD4d3+4-TMEM24

Plasmid

#73092

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

TMEM24 (C2CD2L Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pY008 (pcDNA3.1-hPbCpf1)

Plasmid

#69980

Purpose

Expresses humanized LPbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
POPDC2 pEGFP-N2 (1087)

Plasmid

#62030

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

POPDC2 (POPDC2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only