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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMIR-R/Tac1/SG

Plasmid

#16296

Depositor

Pranela Rameshwar

Article

Greco et al Proc Natl Acad Sci U S A. 2007 Sep 25. 1

Insert

Tac1 3'UTR (TAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pJUMP39-1A(sfGFP)

Plasmid

#126983

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)

Plasmid

#174527

Purpose

Dual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-EGFP-2xmiR-1 (pAW-AS-21)

Plasmid

#40832

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Two target sites perfectly complementary to miR-1

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pJUMP27ts-1A(lacZ)

Plasmid

#126977

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-335 site

Plasmid

#25028

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-335 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
Pconst-rfp + PmutS-yfp

Plasmid

#121444

Purpose

plasmid reporting the expression from constitutive and mutS promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
Pconst-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

P-const and P-mutS

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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PacrAB-rfp + Pconst-yfp

Plasmid

#121445

Purpose

plasmid reporting the expression from acrAB and constitutive promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

Pconst-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and Pconst

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F2 LUC

Plasmid

#22428

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F2 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Available Since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pHU6_Ch13_102010574-gRNA-for

Plasmid

#81218

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDM_noro_A1_lacZA

Plasmid

#118818

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A1

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-mCherryBb

Plasmid

#149636

Purpose

Psyn-mCherry promoter strength reporter for use in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

Psyn-mCherryBb

Tags

mCherry

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAN-PA3-RFP

Plasmid

#62249

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA3

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSB1C3-hsg(M)

Plasmid

#138262

Purpose

Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

sgRNA M

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1-mutant Fzd3 3'UTR

Plasmid

#25023

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant Fzd3 3'UTR

Use

Luciferase

Expression

Mammalian

Mutation

miR-31 binding site in 3’ UTR mutagenized from TC…

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pJUMP47ts-2A(sfGFP)

Plasmid

#126992

Purpose

Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Pou2f3

Plasmid

#84321

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pou2f3 (Pou2f3 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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