We narrowed to 17,206 results for: puro

1. FLAG-TKFC in pLJM60

   Plasmid

   #154206

   Purpose

   Lentiviral vector for stable expression of TKFC

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   TKFC (DAK Human)
   

   Use

   Lentiviral

   Tags

   N terminal FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p23-NES-AdmCas13d-msfGFP-NES-Flag

   Plasmid

   #165075

   Purpose

   overexpression of AdmCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   AdmCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiCRISPRv1_UQCRC2

   Plasmid

   #177982

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting UQCRC2 (UQCRC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-mCherry-SPASTIN

   Plasmid

   #180647

   Purpose

   Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, has siRNA resistance to GAACAGUGUG…

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR-v1-sgHK2_2

   Plasmid

   #163461

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting HK2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FEN1 C9.3 gRNA

   Plasmid

   #90688

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FEN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   FEN1 (Guide Designation C9.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDECKO-mCherry GFP

   Plasmid

   #78535

   Purpose

   Control plasmid (paired gRNAs against GFP)

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BII-ChPtW-EGFP

   Plasmid

   #133400

   Purpose

   Piggybac vector for constitutive expression of EGFP

   Depositor

   Blair Madison

   Article

   Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiCRISPRv2 EGFP sgRNA1

   Plasmid

   #164932

   Purpose

   Expresses EGFP sgRNA1 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-IP GFP-Omp25

    Plasmid

    #38249

    Depositor

    Noboru Mizushima

    Article

    Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

    Insert

    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    plasmid contains AA109-145 of rat Omp25/Synj2bp

    Promoter

    Available since

    Sept. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-shLacZ-1735

    Plasmid

    #115454

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shLacZ-1735
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLENTI_RPS2

    Plasmid

    #127127

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS2 (RPS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5FRT PUR mGSK3A K148A

    Plasmid

    #35158

    Depositor

    Jim Woodgett

    Article

    Doble et al Dev Cell. 2007 Jun;12(6):957-71.

    Insert

    GSK-3α (Gsk3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K148A (dominant negative)

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. lentiCRISPR v2-sgMyc_1

    Plasmid

    #138324

    Purpose

    Express guide RNA 2 for mouse Myc

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    cMyc sgRNA (Myc Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. stabilized HP35 tension sensor module

    Plasmid

    #101251

    Purpose

    Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-HP35st-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW-OVOL2-HA

    Plasmid

    #184737

    Purpose

    Doxycycline-inducible lentiviral expression of OVOL2

    Depositor

    Alessia Ciarrocchi, Gloria Manzotti

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Insert

    ovo like zinc finger 2 (OVOL2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-RNF68

    Plasmid

    #126545

    Purpose

    Expresses mCherry-tagged RNF68 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LIN52 D6.4 gRNA

    Plasmid

    #90735

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LIN52

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    LIN52 (Guide Designation D6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.shHmga2

    Plasmid

    #32399

    Depositor

    Monte Winslow, Tyler Jacks

    Article

    Winslow et al Nature. 2011 May 5. 473(7345):101-4.

    Insert

    shRNA targetting Hmga2 (Hmga2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rad51AP1 B12.3 gRNA

    Plasmid

    #90869

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human Rad51AP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    Rad51AP1 (Guide Designation B12.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLPC-NMYC-mTRF1

    Plasmid

    #64162

    Purpose

    Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    mTRF1 (Terf1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pTarget TMEM97-P2A-mCardinal

    Plasmid

    #157747

    Purpose

    Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. FLAG-HA-RNF68

    Plasmid

    #126548

    Purpose

    Expresses FLAG-HA-tagged RNF68 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSMP-EZH1_1

    Plasmid

    #36359

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

25. sgMLST8_1

    Plasmid

    #124905

    Purpose

    Targeting human MLST8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CHEK2 C3.2 gRNA

    Plasmid

    #90628

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CHEK2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CHEK2 (Guide Designation C3.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. IRAK1mut-UTR

    Plasmid

    #15096

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    IRAK1 3'UTR (IRAK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    4-bp substitutions in the miR-146 target sites

    Promoter

    Available since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pSMP-Bmi1_1

    Plasmid

    #36352

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RRM1 E1.3 gRNA

    Plasmid

    #90880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LPCX BMP-RIB Q203D

    Plasmid

    #12642

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RIB (Bmpr1b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D, activating mutation

    Promoter

    Available since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

32. 5K-tails-->R GLUT1-GFP

    Plasmid

    #200099

    Purpose

    human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 6, 7, 451, 456,…

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEBTet-SNAP-CCCAP

    Plasmid

    #136798

    Purpose

    Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CCCAP (SDCCAG8 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MO70-mJP-4

    Plasmid

    #79598

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

    Insert

    Junctophilin-4 (Jph4 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

35. MSCV P2Gm p53

    Plasmid

    #19751

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. lentiCRISPR v2-sgMyc_2

    Plasmid

    #138346

    Purpose

    Express guide RNA 1 for mouse Myc

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    cMyc sgRNA (Myc Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. BRCA1 A9.2 gRNA

    Plasmid

    #90548

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A9.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mADAM10 E385A in pQCXIP cFLAG

    Plasmid

    #19137

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM10 (Adam10 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E385A

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

39. LENTICRISPR-PGAM1_sgRNA1

    Plasmid

    #201614

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PPP2R2B B6.5 gRNA

    Plasmid

    #90848

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP2R2B

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP2R2B (Guide Designation B6.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge