We narrowed to 11,451 results for: Vars

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-oScarlet

Plasmid

#220606

Purpose

Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

oScarlet

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1_RAF1 (51-131)

Plasmid

#119218

Purpose

purification of GST-RAF1 RBD from E. coli

Depositor

Helen Morrison

Article

Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

Insert

RAF1 aa 51-131 (RAF1 Human)

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Mutation

Codon Optimized

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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SUV[SET]-dCas9

Plasmid

#100088

Purpose

Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

SUVAR39H1 (SUV39H1 Human)

Use

CRISPR

Tags

3XFLag-NLS-SUV[SET]-dCas9-NLS

Expression

Mammalian

Mutation

deleted aa 1-76

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44s (-cyt)

Plasmid

#137815

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44s (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

Plasmid

#101064

Purpose

AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2_L398T

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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EGFR (del3) L747-E749del, A750P

Plasmid

#11015

Purpose

Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (del3) (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L747-E749 deleted, A750P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
GB1-A11(2-196, D40G)-Strep

Plasmid

#196210

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

D40G

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE

Plasmid

#73438

Purpose

Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184S

Plasmid

#106198

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G38R)-Strep

Plasmid

#196209

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G38R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TAF4B

Plasmid

#192927

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TAF4B

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TAF4B (TAF4B Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HDM_Spikedelta21_sinobiological_B.1.617.2

Plasmid

#204149

Purpose

Expression plasmid for SARS-CoV-2 Delta variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 Delta variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNMSB104

Plasmid

#199314

Purpose

MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR

Expression

Worm

Mutation

mTagBFP2 from pJJR81, C. elegans codon optimized …

Promoter

flp-18

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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